• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

源自DNA序列的pBR322限制酶切图谱：长达4361个核苷酸对的精确DNA大小标记。

pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

作者信息

Sutcliffe J G

出版信息

Nucleic Acids Res. 1978 Aug;5(8):2721-8. doi: 10.1093/nar/5.8.2721.

DOI:10.1093/nar/5.8.2721
PMID:358147
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342202/
Abstract

I have derived a complete restriction map of pBR322 from the total nucleotide sequence of the plasmid. Most of the restriction sites also have been demonstrated empirically. The exact sizes of all restriction fragments and the relative positions of the cuts are presented. These fragments can serve as accurate DNA size markers from small pieces up to the 4362 base pair length of pBR322. Inserts cloned in this vector may be characterized easily using this data.

摘要

我已根据该质粒的全核苷酸序列推导出了pBR322的完整限制酶切图谱。大多数限制酶切位点也已通过实验得到证实。文中给出了所有限制酶切片段的确切大小以及酶切位点的相对位置。这些片段可作为精确的DNA大小标记，范围从小片段到pBR322的4362个碱基对长度。利用这些数据，克隆到该载体中的插入片段可轻松进行特征鉴定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bccb/342202/552191692b0d/nar00469-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bccb/342202/552191692b0d/nar00469-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bccb/342202/552191692b0d/nar00469-0043-a.jpg

相似文献

1
pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.源自DNA序列的pBR322限制酶切图谱：长达4361个核苷酸对的精确DNA大小标记。
Nucleic Acids Res. 1978 Aug;5(8):2721-8. doi: 10.1093/nar/5.8.2721.
2
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.大肠杆菌质粒pBR322的完整核苷酸序列。
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90. doi: 10.1101/sqb.1979.043.01.013.
3
A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322.用于质粒载体pBR322中DNA测序的一组合成寡脱氧核糖核苷酸引物。
Gene. 1981 Dec;16(1-3):21-6. doi: 10.1016/0378-1119(81)90057-3.
4
Nucleotide sequence of Escherichia coli K-12 replication origin.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9. doi: 10.1073/pnas.76.2.575.
5
Physical map of the nrdA-nrdB-ftsB-glpT region of the chromosomal DNA of Escherichia coli.大肠杆菌染色体DNA的nrdA - nrdB - ftsB - glpT区域的物理图谱。
Gene. 1982 Jun;18(3):309-18. doi: 10.1016/0378-1119(82)90169-x.
6
A subcloning strategy for DNA sequence analysis.一种用于DNA序列分析的亚克隆策略。
Nucleic Acids Res. 1980 Dec 11;8(23):5541-9. doi: 10.1093/nar/8.23.5541.
7
Preferential nucleosome placement on pBR322 restriction fragments.核小体在pBR322限制性片段上的优先定位
Biochem Biophys Res Commun. 1986 Jul 16;138(1):110-7. doi: 10.1016/0006-291x(86)90253-6.
8
Replication and expression of fragments of phage PM2 cloned in Escherichia coli K-12.在大肠杆菌K-12中克隆的噬菌体PM2片段的复制与表达。
Acta Microbiol Pol. 1981;30(4):307-18.
9
The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R1drd19.来自抗生素抗性因子R1drd19复制起点的一段DNA片段的核苷酸序列。
Mol Gen Genet. 1979 Mar 27;171(3):277-85. doi: 10.1007/BF00267582.
10
[A method for inducing the point C----T transitions in DNA sequences corresponding to the ends of restriction sites].一种在与限制性酶切位点末端相对应的DNA序列中诱导C----T点突变的方法
Bioorg Khim. 1988 May;14(5):694-6.

引用本文的文献

1
Hamster Polyomavirus Research: Past, Present, and Future.仓鼠多瘤病毒研究：过去、现在和未来。
Viruses. 2021 May 13;13(5):907. doi: 10.3390/v13050907.
2
Cellular responses during morphological transformation in Azospirillum brasilense and Its flcA knockout mutant.巴西固氮螺菌及其flcA基因敲除突变体形态转变过程中的细胞反应。
PLoS One. 2014 Dec 12;9(12):e114435. doi: 10.1371/journal.pone.0114435. eCollection 2014.
3
Mitochondrial plasmid-like molecules in fertile and male sterileVicia faba L.蚕豆可育和不育株中线粒体质粒样分子

本文引用的文献

1
Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.质粒ColE1作为用于DNA克隆和扩增的分子载体。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9. doi: 10.1073/pnas.71.9.3455.
2
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.利用分析型琼脂糖-溴化乙锭电泳检测副流感嗜血杆菌中的两种限制性内切酶活性。
Biochemistry. 1973 Jul 31;12(16):3055-63. doi: 10.1021/bi00740a018.
3
Nucleotide sequence of bacteriophage phi X174 DNA.噬菌体φX174 DNA的核苷酸序列。
Plant Mol Biol. 1983 Nov;2(6):305-9. doi: 10.1007/BF01578591.
4
The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii.在莱茵衣藻中，RuBP 羧化酶/加氧酶小亚基的两个基因紧密连锁。
Plant Mol Biol. 1986 Jan;6(1):13-21. doi: 10.1007/BF00021302.
5
Evidence for transcriptional regulation of dihydroorotic acid dehydrogenase in Saccharomyces cerevisiae.酵母中二氢乳清酸脱氢酶转录调控的证据。
Curr Genet. 1981 May;3(2):119-23. doi: 10.1007/BF00365715.
6
MboI, ThaI and HinfI endonuclease cleavage maps of the yeast mitochondrial DNA : Localization of transfer RNA genes.酵母线粒体 DNA 的 MboI、ThaI 和 HinfI 内切酶酶切图谱：转移 RNA 基因的定位。
Curr Genet. 1980 Oct;2(2):121-9. doi: 10.1007/BF00420624.
7
Transformation of Saccharomyces cerevisiae with plasmids containing fragments of yeast 2 μ DNA and a suppressor tRNA gene.用含有酵母 2 μ DNA 片段和一个抑制 tRNA 基因的质粒转化酿酒酵母。
Curr Genet. 1980 Jul;2(1):9-16. doi: 10.1007/BF00445689.
8
Mapping of mitochondrial DNA from Torulopsis glabrata : Location of ribosomal and transfer RNA genes.从光滑球拟酵母中绘制线粒体 DNA 图谱：核糖体和转移 RNA 基因的位置。
Curr Genet. 1980 Apr;1(3):209-17. doi: 10.1007/BF00390946.
9
Does mitochondrial DNA length influence the frequency of spontaneous petite mutants in yeasts?线粒体 DNA 长度是否影响酵母中自发 petite 突变体的频率？
Curr Genet. 1981 Sep;4(1):7-12. doi: 10.1007/BF00376779.
10
Highlights of the DNA cutters: a short history of the restriction enzymes.DNA 剪刀的亮点：限制酶的简史。
Nucleic Acids Res. 2014 Jan;42(1):3-19. doi: 10.1093/nar/gkt990. Epub 2013 Oct 18.
Nature. 1977 Feb 24;265(5596):687-95. doi: 10.1038/265687a0.
4
A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.一种来自嗜热支原体嗜酸热原体的限制酶Tha I。
Nucleic Acids Res. 1978 Jun;5(6):1729-39. doi: 10.1093/nar/5.6.1729.
5
Detection of 5-methylcytosine in DNA sequences.DNA序列中5-甲基胞嘧啶的检测。
Nucleic Acids Res. 1978 May;5(5):1479-85. doi: 10.1093/nar/5.5.1479.
6
A new method for sequencing DNA.一种新的DNA测序方法。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.
7
Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12.大肠杆菌K12中DNA腺嘌呤甲基化突变（dam-3）的多效性作用
Mutat Res. 1975 Apr;28(1):15-26. doi: 10.1016/0027-5107(75)90309-7.
8
The cleavage site of the restriction endonuclease Ava II.限制性内切酶Ava II的切割位点。
Nucleic Acids Res. 1978 Jul;5(7):2313-9. doi: 10.1093/nar/5.7.2313.