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使用 Folin-Denis 和蛋白质沉淀两种技术比较两个梨品种发育果芽中的单宁含量。

Comparison of tannin levels in developing fruit buds of two orchard pear varieties using two techniques, Folin-Denis and protein precipitation assays.

机构信息

Agricultural Science Service, Tolworth Laboratory Ministry of Agriculture, Fisheries and Food Hook Rise South, Tolworth, KT6 7NF, Surbiton, Surrey, UK.

出版信息

J Chem Ecol. 1984 Mar;10(3):493-8. doi: 10.1007/BF00988095.

DOI:10.1007/BF00988095
PMID:24318554
Abstract

As part of a study into dietary selection by avian pest species, the levels of phenolic material in pear buds have been determined using two approaches, the Folin-Denis assay and an assay based on the precipitation of bovine serum albumin by astringent phenols. The results of these two assays do not correlate over the sampling period and their overall significance to ecological studies is discussed.

摘要

作为鸟类害虫物种饮食选择研究的一部分，使用两种方法（福林-丹尼斯法和基于丹宁酸沉淀牛血清白蛋白的方法）测定了梨芽中酚类物质的水平。这两种方法的测定结果在采样期间没有相关性，并且讨论了它们对生态研究的总体意义。

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本文引用的文献

1
Tannin assays in ecological studies: Lack of correlation between phenolics, proanthocyanidins and protein-precipitating constituents in mature foliage of six oak species.生态研究中的单宁测定：六种栎属植物成熟叶片中酚类物质、原花青素与蛋白质沉淀成分之间缺乏相关性。
Oecologia. 1982 Aug;54(2):205-211. doi: 10.1007/BF00378394.
2
Tannin assays in ecological studies Precipitation of ribulose-1,5-bisphosphate carboxylase/oxygenase by tannic acid, quebracho, and oak foliage extracts.鞣酸、奎那酸和橡树叶提取物对核酮糖-1,5-二磷酸羧化酶/加氧酶的沉淀作用：生态研究中的丹宁分析。
J Chem Ecol. 1983 Feb;9(2):285-94. doi: 10.1007/BF00988046.
使用染料标记的蛋白质作为分光光度法测定蛋白质沉淀剂（如单宁）的方法。
J Chem Ecol. 1985 Nov;11(11):1535-44. doi: 10.1007/BF01012199.
4
Bullfinches and ash trees : Assessing the role of plant chemicals in controlling damage by herbivores.松雀和白蜡树：评估植物化学物质在控制食草动物损害中的作用。
J Chem Ecol. 1988 Oct;14(10):1889-903. doi: 10.1007/BF01013484.