Department of Biological Sciences and Marine Sciences Institute, University of California, 93106, Santa Barbara, California.
J Chem Ecol. 1984 Jun;10(6):957-71. doi: 10.1007/BF00987976.
Molecular weight fractions of abalone muscle were tested for the ability to induce appetitive feeding and locomotor behavior in the spiny lobster,Panulirus interruptus. Fractions of <1000, 1000-10,000 and >10,000 daltons were isolated by ultrafiltrations and gel chromatography from a seawater extract of abalone muscle. The two lower-molecular-weight fractions (<1000, 1000-10,000) were the least stimulatory of the three fractions tested, and both were ineffective as feeding stimulants. Solutions combining any two of the three isolated fractions produced behavioral activity equal to that caused by whole extract; thus, no single fraction was essential to the stimulatory capacity of abalone. The >1000-dalton fraction was also highly stimulatory, meaning that large and not small molecules were essential in initiating feeding. Finally, a 75% ethanol-insoluble component of the <10,000 fraction was effective, while the ethanol-soluble portion was not. Since the insoluble material consisted predominantly of peptides and polypeptides, it is probable that these molecules act as principal stimulants in abalone muscle.
从鲍鱼肌肉的海水提取物中，通过超滤和凝胶色谱法分离出<1000、1000-10000 和>10000 道尔顿的分子量级分。在测试的三个级分中，两个较低分子量级分（<1000、1000-10000）的刺激作用最小，两者均不能作为摄食刺激物。将三种分离级分中的任意两种组合的溶液可产生与整个提取物相同的行为活性；因此，没有任何单一级分对于鲍鱼的刺激能力是必不可少的。>1000 道尔顿级分也具有高度的刺激性，这意味着引发摄食的是大分子而不是小分子。最后，<10000 级分的 75%乙醇不溶性成分有效，而乙醇可溶部分无效。由于不溶性物质主要由肽和多肽组成，因此这些分子很可能是鲍鱼肌肉中的主要刺激物。
