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大鼠胚胎细胞用于体内再生治疗的安全性评估。

Safety assessment of rat embryonic fraction for in vivo regenerative therapy.

机构信息

Department of Biotechnology, School of Life Sciences, Gitam University, Visakhapatnam, AP, India530045.

Department of Biochemistry, University of Hyderabad, Hyderabad, TG, India500046.

出版信息

Biol Open. 2024 Aug 15;13(8). doi: 10.1242/bio.060266. Epub 2024 Aug 22.

DOI:10.1242/bio.060266
PMID:38984587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11360137/
Abstract

Regenerative therapy is considered a novel option for treating various diseases, whereas a developing embryo is a prime source of molecules that help repair diseased tissue and organs. Organoid culture studies also confirmed the inherent biological functions of several embryonic factors. However, the in vivo safety and efficacy of embryonic protein fraction (EPF) were not validated. In this study, we investigated the effectiveness of EPF on healthy adult rats. We obtained embryos from Sprague-Dawley (SD) female rats of E14, E16, and E19 embryonic days and collected protein lysate. This lysate was administered intravenously into adult SD rats on sequential days. We collected blood and performed hematological and biochemical parameters of rats that received EPF. C-reactive protein levels, interleukin-6, blood glucose levels, serum creatinine, blood urea, total leucocyte counts, and % of neutrophils and lymphocytes were comparable between rats receiving EPF and saline. Histological examination of rats' tissues administered with EPF is devoid of abnormalities. Our study revealed that intravenous administration of EPF to healthy adult rats showed that EPF is non-immunogenic, non-inflammatory, non-tumorigenic, and safe for in vivo applications. Our analysis suggests that EPF or its components could be recommended for validating its therapeutic abilities in organ regenerative therapy.

摘要

再生疗法被认为是治疗各种疾病的一种新选择，而发育中的胚胎是帮助修复患病组织和器官的分子的主要来源。类器官培养研究也证实了几种胚胎因子的固有生物学功能。然而，胚胎蛋白提取物 (EPF) 的体内安全性和疗效尚未得到验证。在这项研究中，我们研究了 EPF 对健康成年大鼠的有效性。我们从 E14、E16 和 E19 天的 Sprague-Dawley (SD) 雌性大鼠中获得胚胎，并收集蛋白裂解物。将这种裂解物连续几天静脉内给予成年 SD 大鼠。我们收集血液并对接受 EPF 的大鼠进行血液学和生化参数检测。接受 EPF 和生理盐水的大鼠的 C 反应蛋白水平、白细胞介素 6、血糖水平、血清肌酐、血尿素、总白细胞计数以及中性粒细胞和淋巴细胞的百分比均无差异。给予 EPF 的大鼠组织的组织学检查无异常。我们的研究表明，向健康成年大鼠静脉内给予 EPF 表明 EPF 具有非免疫原性、非炎症性、非肿瘤性，并且可安全用于体内应用。我们的分析表明，EPF 或其成分可被推荐用于验证其在器官再生治疗中的治疗能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a93/11360137/c10bccdb33d8/biolopen-13-060266-g7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a93/11360137/c10bccdb33d8/biolopen-13-060266-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a93/11360137/de5e5e95f10c/biolopen-13-060266-g1.jpg
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