Rustan A C, Nossen J O, Blomhoff J P, Drevon C A
Int J Biochem. 1986;18(10):909-16. doi: 10.1016/0020-711x(86)90072-8.
Primary cultures of rat hepatocytes were used to study the release of hepatic lipase and very low density lipoprotein (VLDL). The presence of hepatic lipase activity was proved by salt-resistance, affinity chromatography and inactivation by a hepatic lipase antibody. Cellular rate of hepatic lipase release increased by prolonged time in culture, whereas VLDL secretion decreased. Oleic acid and dextran-70 had no effect on release of hepatic lipase, whereas VLDL secretion was increased and decreased, respectively. Calcium antagonists (cobalt and verapamil), monensin and cycloheximide inhibited both the release of hepatic lipase and VLDL. Colchicine and chloroquine, which decreased VLDL secretion, had no effect on release of hepatic lipase. The present results suggest that release of hepatic lipase and secretion of VLDL are not coordinated and exhibit different sensitivity towards certain compounds altering secretory functions.
利用大鼠肝细胞原代培养物来研究肝脂肪酶和极低密度脂蛋白（VLDL）的释放。通过耐盐性、亲和层析以及肝脂肪酶抗体使其失活，证实了肝脂肪酶活性的存在。随着培养时间延长，肝脂肪酶的细胞释放速率增加，而VLDL分泌减少。油酸和右旋糖酐-70对肝脂肪酶的释放没有影响，而VLDL分泌则分别增加和减少。钙拮抗剂（钴和维拉帕米）、莫能菌素和环己酰亚胺同时抑制肝脂肪酶的释放和VLDL的分泌。秋水仙碱和氯喹可减少VLDL分泌，但对肝脂肪酶的释放没有影响。目前的结果表明，肝脂肪酶的释放和VLDL的分泌不协调，并且对某些改变分泌功能的化合物表现出不同的敏感性。
