Küpper J D, Brandt H R, Erhardt G
Institut für Tierzucht und Haustiergenetik, Justus-Liebig-Universität, Gießen, 35390, Germany.
Anim Genet. 2014 Feb;45(1):114-6. doi: 10.1111/age.12097. Epub 2013 Dec 10.
The aim of the study was the analysis of the nucleotide-binding oligomerization domain containing 2 (NOD2, formerly CARD15) as a candidate gene for Mycobacterium avium ssp. paratuberculosis infection in cattle. Eleven SNPs in the NOD2 gene were identified, and finally, four SNPs were included in a case-control study using 324 German Holstein cows tested for paratuberculosis using fecal culture and ELISA. The SNP (GenBank) AY518738S04:g.521G>A in exon 4 showed a significant association between the fecal culture status of the animals and NOD2 allele variants. The other three SNPs showed no associations in German Holstein cows.
本研究的目的是分析含核苷酸结合寡聚化结构域2（NOD2，原名CARD15）作为牛副结核分枝杆菌感染候选基因的情况。在NOD2基因中鉴定出11个单核苷酸多态性（SNP），最终，在一项病例对照研究中纳入了4个SNP，该研究使用324头经粪便培养和酶联免疫吸附测定（ELISA）检测副结核的德国荷斯坦奶牛。第4外显子中的SNP（GenBank登录号AY518738S04:g.521G>A）显示动物的粪便培养状况与NOD2等位基因变体之间存在显著关联。其他3个SNP在德国荷斯坦奶牛中未显示出关联。
