a Department of Protein Chemistry and Technology , CSIR-Central Food Technological Research Institute , Mysore , India.
Prep Biochem Biotechnol. 2014;44(4):392-417. doi: 10.1080/10826068.2013.833108.
The indigenous bacteria Bacillus sp. CFR1601 produced significant levels of endo-mannanase when grown on agro-wastes, namely, green gram husk and sunflower oil cake (25.6 IU/mL), used as sole carbon and nitrogen sources, respectively. Under immobilized cell system, synthetic supports (polyurethane foam, scotch brite, polyester; up to 33.2 IU/mL) were found marginally superior as compared to natural supports (cotton and silk; up to 28.2 IU/mL) for endo-mannanase production. Cooperative interactions between L-lysine HCl (0.3% w/v), Tween 60 (0.3% v/v), and sunflower oil cake (3.0% w/v) in central composite design response surface methodology ameliorated (1.61-fold) endo-mannanase titers to 48.0 IU/mL. Partially purified endo-mannanase was tested for its ability to produce oligosaccharides from guar gum. These oligosaccharides were tested in vitro for their ability to promote growth of Lactobacillus plantarum MTCC 5422 and Lactobacillus salivarius CHS 1E. Results indicated that low-molecular-weight degraded products from guar gum were (1) able to support the growth of tested strains [increased O.D600nm up to 2.3-fold and decrease in pH (<6.3) due to production of short chain fatty acid (SCFA)] when used as sole carbon source; and (2) after purification and analysis by electron spray ionization-mass spectrometry (ESI-MS) were found to be composed of mainly disaccharide and tetrasaccharide. The compatibility of endo-mannanase with various detergents together with wash performance test confirmed its potential applicability for laundry industry.
当以农业废弃物（绿豆皮和葵花油饼，分别作为唯一的碳源和氮源）为培养物时，土著细菌 Bacillus sp. CFR1601 大量产生内切甘露聚糖酶。在固定化细胞系统中，与天然载体（棉和丝，最高分别为 28.2IU/mL）相比，合成载体（聚氨酯泡沫、斯考特布赖特、聚酯，最高分别为 33.2IU/mL）更有利于内切甘露聚糖酶的产生。在中心组合设计响应面法中，L-赖氨酸 HCl（0.3%w/v）、吐温 60（0.3%v/v）和葵花油饼（3.0%w/v）的协同作用将内切甘露聚糖酶的酶活提高了（1.61 倍），达到 48.0IU/mL。部分纯化的内切甘露聚糖酶被测试其从瓜尔胶产生低聚糖的能力。这些低聚糖在体外被测试其促进植物乳杆菌 MTCC 5422 和唾液乳杆菌 CHS 1E 生长的能力。结果表明，瓜尔胶的低分子量降解产物（1）能够支持测试菌株的生长[增加 O.D600nm 高达 2.3 倍，由于短链脂肪酸（SCFA）的产生，pH 值降低（<6.3）]，当用作唯一的碳源时；（2）经纯化和电子喷雾电离质谱（ESI-MS）分析，主要由二糖和四糖组成。内切甘露聚糖酶与各种洗涤剂的相容性以及洗涤性能测试证实了其在洗衣行业的潜在适用性。
