Nomura S, Itoh K, Sugimoto T, Yasui Y, Kamiya H, Mizuno N
J Comp Neurol. 1986 Nov 1;253(1):121-33. doi: 10.1002/cne.902530110.
Somatotopic arrangements of axon terminals of primary afferent fibers innervating follicles of the mystacial vibrissae were examined in the cat by the transganglionic horseradish peroxidase (HRP) method. Forty to 60 hours after injecting HRP into a single or a group of vibrissal follicles, transported HRP was visualized by the tetramethylbenzidine technique. HRP-labeled axon terminals were distributed in the ventral subnucleus of the principal sensory trigeminal nucleus (ventral Vp), in the oral and interpolar spinal trigeminal nuclei (Vo and Vi), and in the caudal spinal trigeminal nucleus (Vc) (layer I, deep part of layer II, layers III-V) with its spinal extension into the dorsal horn of the first cervical cord segment (rostral C1). In cross sections through the caudal parts of the ventral Vp, Vi, and layer IV of the Vc and rostral C1, a single mystacial vibrissa was represented in a one-to-one fashion by a patch of dense terminal arbors of primary afferent fibers. The more dorsally a horizontal row of the mystacial vibrissae was located, the more ventrally was it represented in the ventral Vp, the more ventrolaterally in the Vi, and the more ventrally in layer IV of the Vc and the rostral C1. In addition, the more anteriorly a vibrissa was located in a horizontal row of the mystacial vibrissae, the more medially was it represented in the ventral Vp, the more ventromedially in the Vi, and the more laterally in layer IV of the Vc and rostral C1; the most posteriorly located vibrissae in the horizontal rows of the mystacial vibrissae were represented along the lateral border of the ventral Vp and Vi, and most medially in layer IV of the Vc and rostral C1. Thus, the representation pattern in the ventral Vp was rotated clockwise at about 45 degrees angle in the Vi, and projected as a mirror image in layer IV of the Vc and rostral C1. It was also indicated that the anterior-posterior arrangement of the mystacial vibrissae was represented in a rostral-caudal organization within layer IV of the Vc and rostral C1. It was also indicated that the anterior-posterior arrangement of the mystacial vibrissae was represented in a rostral-caudal organization within layer IV of the Vc and rostral C1. Patchy patterns probably replicating the distribution of the vibrissae on the face of the cat were also revealed by the cytochrome oxidase histochemical staining in cross sections through the caudal parts of the ventral Vp, Vi, and layer IV of the Vc and rostral C1.
运用跨神经节辣根过氧化物酶（HRP）法，在猫身上研究了支配触须毛囊的初级传入纤维轴突终末的躯体定位排列。将HRP注入单个或一组触须毛囊40至60小时后，采用四甲基联苯胺技术使转运的HRP可视化。HRP标记的轴突终末分布在三叉神经感觉主核的腹侧亚核（腹侧Vp）、口部和极间脊髓三叉神经核（Vo和Vi）以及尾侧脊髓三叉神经核（Vc）（I层、II层深部、III - V层），其脊髓延伸部分进入第一颈髓节段的背角（头端C1）。在通过腹侧Vp、Vi的尾侧部分以及Vc和头端C1的IV层的横切面上，一根触须由初级传入纤维的一片密集终末分支以一对一的方式代表。触须水平排越靠背侧，在腹侧Vp中代表的位置越靠腹侧，在Vi中越靠腹外侧，在Vc的IV层和头端C1中越靠腹侧。此外，触须在触须水平排中越靠前，在腹侧Vp中代表的位置越靠内侧，在Vi中越靠腹内侧，在Vc的IV层和头端C1中越靠外侧；触须水平排中最靠后的触须沿着腹侧Vp和Vi的外侧缘代表，在Vc的IV层和头端C1中最靠内侧。因此，腹侧Vp中的代表模式在Vi中顺时针旋转约45度角，并在Vc的IV层和头端C1中呈镜像投射。还表明触须的前后排列在Vc的IV层和头端C1内以头 - 尾组织形式代表。通过细胞色素氧化酶组织化学染色，在通过腹侧Vp、Vi的尾侧部分以及Vc和头端C1的IV层的横切面上也揭示了可能复制猫面部触须分布的斑片状模式。
