Yukl Steven A, Sinclair Elizabeth, Somsouk Ma, Hunt Peter W, Epling Lorrie, Killian Maudi, Girling Valerie, Li Peilin, Havlir Diane V, Deeks Steven G, Wong Joseph K, Hatano Hiroyu
aSan Francisco Veterans Affairs Medical Center bUniversity of California, San Francisco (UCSF), California, USA.
AIDS. 2014 Jan 28;28(3):439-42. doi: 10.1097/QAD.0000000000000166.
We compared different techniques for measuring gut HIV reservoirs and assessed for HIV in non-CD4 T cells. HIV DNA levels were similar when measured from rectal biopsies and isolated rectal cells, while HIV RNA tended to be higher in rectal cells. HIV DNA levels in total rectal cells were greater than those predicted from levels in sorted CD4 T cells, suggesting a reservoir in non-CD4 T cells, and HIV DNA was detected in sorted myeloid cells (7/7 subjects).
我们比较了测量肠道HIV储存库的不同技术，并评估了非CD4 T细胞中的HIV情况。从直肠活检组织和分离出的直肠细胞中测量时，HIV DNA水平相似，而直肠细胞中的HIV RNA往往更高。总直肠细胞中的HIV DNA水平高于从分选的CD4 T细胞水平预测的值，这表明在非CD4 T细胞中存在储存库，并且在分选的髓样细胞中检测到了HIV DNA（7/7名受试者）。
