Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Nanoscale. 2014;6(3):1552-9. doi: 10.1039/c3nr05273g.
The monitoring of grafted stem cells is crucial to assess the efficiency, effectiveness and safety of such stem cell-based therapies. In this regard, a reliable and cytocompatible labeling method for stem cells is critically needed. In this study, the collagen-functionalized single-walled carbon nanotubes (Col-SWCNTs) were used as imaging probes for labeling of human mesenchymal stem cells (hMSCs) and the inherent Raman scattering of SWCNTs was used to image the SWCNT-labeled cells. The results showed that the Col-SWCNTs exhibit efficient cellular internalization by hMSCs without affecting their proliferation and differentiation. The prolonged dwell time of Col-SWCNTs in cells ensured the long-term labeling for up to 2 weeks. This work reveals the potential of Col-SWCNTs as probes for long-term stem cell labeling.
对移植干细胞的监测对于评估基于干细胞的治疗方法的效率、效果和安全性至关重要。在这方面，迫切需要一种可靠且与细胞相容的干细胞标记方法。在这项研究中，胶原功能化单壁碳纳米管（Col-SWCNTs）被用作人骨髓间充质干细胞（hMSCs）的成像探针，并且SWCNTs 的固有拉曼散射被用于对 SWCNT 标记的细胞进行成像。结果表明，Col-SWCNTs 可以有效地被 hMSCs 内化，而不会影响其增殖和分化。Col-SWCNTs 在细胞中的停留时间延长，确保了长达 2 周的长期标记。这项工作揭示了 Col-SWCNTs 作为长期干细胞标记探针的潜力。
