Department of Molecular Science and Technology, Ajou University , Suwon 443-749, Republic of Korea.
ACS Appl Mater Interfaces. 2015 Jan 28;7(3):1560-7. doi: 10.1021/am506833q. Epub 2015 Jan 16.
Studies on the interaction of cells with single-walled carbon nanotubes (SWCNTs) have been receiving increasing attention owing to their potential for various cellular applications. In this report, we investigated the interactions between biological cells and nanostructured SWCNTs films and focused on how morphological structures of SWCNT films affected cellular behavior such as cell proliferation and differentiation. One directionally aligned SWCNT Langmuir-Blodgett (LB) film and random network SWCNT film were fabricated by LB and vacuum filteration methods, respectively. We demonstrate that our SWCNT LB and network film based scaffolds do not show any cytotoxicity, while on the other hand, these scaffolds promote differentiation property of rat mesenchymal stem cells (rMSCs) when compared with that on conventional tissue culture polystyrene substrates. Especially, the SWCNT network film with average thickness and roughness values of 95 ± 5 and 9.81 nm, respectively, demonstrated faster growth rate and higher cell thickness for rMSCs. These results suggest that systematic manipulation of the thickness, roughness, and directional alignment of SWCNT films would provide the convenient strategy for controlling the growth and maintenance of the differentiation property of stem cells. The SWCNT film could be an alternative culture substrate for various stem cells, which often require close control of the growth and differentiation properties.
由于单壁碳纳米管 (SWCNT) 在各种细胞应用方面的潜力，细胞与单壁碳纳米管相互作用的研究受到越来越多的关注。在本报告中，我们研究了生物细胞与纳米结构 SWCNT 薄膜之间的相互作用，并重点研究了 SWCNT 薄膜的形态结构如何影响细胞增殖和分化等细胞行为。通过 Langmuir-Blodgett (LB) 和真空过滤方法分别制备了定向排列的 SWCNT LB 薄膜和随机网络 SWCNT 薄膜。我们证明，我们的 SWCNT LB 和网络薄膜支架没有显示出任何细胞毒性，另一方面，与传统的组织培养聚苯乙烯底物相比，这些支架促进了大鼠间充质干细胞 (rMSCs) 的分化特性。特别是，具有平均厚度和粗糙度值分别为 95 ± 5nm 和 9.81nm 的 SWCNT 网络薄膜，表现出 rMSCs 更快的生长速度和更高的细胞厚度。这些结果表明，系统地操纵 SWCNT 薄膜的厚度、粗糙度和定向排列，可以为控制干细胞的生长和维持分化特性提供简便的策略。SWCNT 薄膜可以作为各种干细胞的替代培养底物，这些干细胞通常需要密切控制其生长和分化特性。
