Reeves B R, Fisher C, Smith S, Courtenay V D, Robertson D
J Natl Cancer Inst. 1987 Jan;78(1):7-18. doi: 10.1093/jnci/78.1.7.
A cell line (RM-HS1) derived from a human epithelioid sarcoma was established in tissue culture. Ultrastructurally, the cells show features of those found within the primary tumor. A mixed mesenchymal-epithelial phenotype, defined by reactivity with antibodies to epithelial membrane antigen and to vimentin and keratin intermediate filaments, was found in the tumor, and a similar phenotype persisted in the cultured cells. Cytogenetic analysis revealed a mode of 66 chromosomes. With the use of a variety of banding techniques together with in situ hybridization of a 3H-labeled molecular probe for 18s and 28s ribosomal RNA genes (pX1r101), the karyotypes were shown to contain extensive numerical and structural rearrangements, with up to 24 marker chromosomes.
从人上皮样肉瘤中分离出的细胞系（RM-HS1）在组织培养中建立。在超微结构上，这些细胞表现出原发性肿瘤细胞的特征。在肿瘤中发现了一种混合的间充质-上皮表型，通过与上皮膜抗原、波形蛋白和角蛋白中间丝抗体的反应性来定义，并且在培养细胞中持续存在类似的表型。细胞遗传学分析显示染色体模式为66条。通过使用各种显带技术以及针对18s和28s核糖体RNA基因（pX1r101）的3H标记分子探针的原位杂交，核型显示包含广泛的数目和结构重排，有多达24条标记染色体。
