Bavariya Ankit J, Andrew Norowski P, Mark Anderson K, Adatrow Pradeep C, Garcia-Godoy Franklin, Stein Sidney H, Bumgardner Joel D
Department of Periodontology, University of Tennessee Health Science Center, College of Dentistry, Memphis, Tennessee.
J Biomed Mater Res B Appl Biomater. 2014 Jul;102(5):1084-92. doi: 10.1002/jbm.b.33090. Epub 2013 Dec 9.
Chitosan, a natural polysaccharide, has demonstrated potential as a degradable biocompatible guided bone regeneration membrane. This study aimed to evaluate the in vivo biocompatibility and degradation of chitosan nanofiber membranes, with and without genipin crosslinking as compared with a commercial collagen membrane in rat model. Chitosan nanofiber membranes, with and without genipin crosslinking, and collagen membrane (control) were implanted subcutaneously in the backs of 30 rats. The membranes were analyzed histologically at 2, 4, 8, 12, 16, and 20 weeks. Sections were viewed and graded by a blinded pathologist using a 4-point scoring system (0 = absent, 1 = mild, 2 = moderate, and 3 = severe) to determine the tissue reaction to the membranes and to observe membrane degradation. There was no statistically significant difference in histological scores among chitosan and collagen membranes at different time points. Absence or minimal inflammation was observed in 57-74% of the membranes across all groups. Most chitosan membranes persisted for 16-20 weeks, whereas most collagen membranes disappeared by resorption at 12-16 weeks. The general tissue response to chitosan nanofiber membranes with and without genipin crosslinking, was similar to that of control commercial collagen membrane. However, the chitosan membranes exhibited slower degradation rates than collagen membranes.
壳聚糖是一种天然多糖，已被证明具有作为可降解生物相容性引导骨再生膜的潜力。本研究旨在评估壳聚糖纳米纤维膜在大鼠模型中的体内生物相容性和降解情况，并将有和没有京尼平交联的壳聚糖纳米纤维膜与市售胶原膜进行比较。将有和没有京尼平交联的壳聚糖纳米纤维膜以及胶原膜（对照）皮下植入30只大鼠的背部。在第2、4、8、12、16和20周对膜进行组织学分析。由一位不知情的病理学家使用4分评分系统（0 = 无，1 = 轻度，2 = 中度，3 = 重度）查看切片并评分，以确定组织对膜的反应并观察膜的降解情况。在不同时间点，壳聚糖膜和胶原膜的组织学评分没有统计学上的显著差异。在所有组中，57 - 74%的膜观察到无炎症或仅有轻微炎症。大多数壳聚糖膜持续存在16 - 20周，而大多数胶原膜在12 - 16周时通过吸收消失。有和没有京尼平交联的壳聚糖纳米纤维膜的一般组织反应与对照市售胶原膜相似。然而，壳聚糖膜的降解速率比胶原膜慢。
