State Key Lab of Pulp and Papermaking Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, China.
State Key Lab of Pulp and Papermaking Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, China.
Carbohydr Polym. 2022 Jun 1;285:119255. doi: 10.1016/j.carbpol.2022.119255. Epub 2022 Feb 17.
Bone tissue repair is difficult due to the dense structure of the extracellular matrix. To solve this problem, a porous chitosan nanofiber scaffold (CSNFS) with an extracellular matrix-like structure was prepared via a facile cross-linked reaction of root-like chitosan nanofiber (CSNF) and collagen (Col) by using genipin (Gen) as the cross-linker. The optimal preparation conditions of CSNFS is weight ratio of CSNF:Col:Gen =1:1:0.1, crosslinked 48 h under 37 °C. CSNFS shows high porosity with adequate micro-scale pores, and its BET data shows that there are a large number of nano-scale pores. The CSNFS mechanical strength is higher than that of the chitosan scaffold both in dry and wet state. MC3T3 cells grow well on CSNFS, can overgrow the scaffold in three-dimensional space, adhere and differentiate well within those nanofiber structure. The cross-linked CSNFS has good biocompatibility and can be used as a repair material for bone tissue engineering.
由于细胞外基质的致密结构，骨组织的修复较为困难。为了解决这个问题，通过使用京尼平（Gen）作为交联剂，通过根状壳聚糖纳米纤维（CSNF）和胶原（Col）的简单交联反应，制备了具有细胞外基质样结构的多孔壳聚糖纳米纤维支架（CSNFS）。CSNFS 的最佳制备条件为 CSNF：Col：Gen 的重量比=1：1：0.1，在 37°C 下交联 48 h。CSNFS 具有较高的孔隙率和充足的微尺度孔，其 BET 数据表明存在大量纳米尺度的孔。CSNFS 的机械强度在干燥和湿润状态下均高于壳聚糖支架。MC3T3 细胞在 CSNFS 上生长良好，可在三维空间中覆盖支架，在纳米纤维结构内良好地黏附和分化。交联的 CSNFS 具有良好的生物相容性，可用作骨组织工程的修复材料。
