Post-Graduate Program in Oral Science (Periodontology Unit), School of Dentistry, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil.
Post-Graduate Program in Oral Science (Pathology Unit), School of Dentistry, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil.
J Biomed Mater Res B Appl Biomater. 2020 Feb;108(2):306-315. doi: 10.1002/jbm.b.34389. Epub 2019 Apr 23.
This study evaluated the biocompatibility of degradable polydioxanone (PDS) electrospun drug delivery systems (hereafter referred as matrices) containing metronidazole (MET) or ciprofloxacin (CIP) after subcutaneous implantation in rats. Sixty adult male rats were randomized into six groups: SHAM (sham surgery); PDS (antibiotic-free matrix); 1MET (one 25 wt% MET matrix); 1CIP (one 25 wt% CIP matrix); 2MET (two 25 wt% MET matrices); and 2CIP (two 25 wt% CIP matrices). At 3 and 30 days, animals were assessed for inflammatory cell response (ICR), collagen fibers degradation, and oxidative profile (reactive oxygen species [ROS]; lipid peroxidation [LP]; and protein carbonyl [PC]). At 3 days, percentages of no/discrete ICR were 100, 93.3, 86.7, 76.7, 50, and 66.6 for SHAM, PDS, 1MET, 1CIP, 2MET, and 2CIP, respectively. At 30 days, percentages of no/discrete ICR were 100% for SHAM, PDS, 1MET, and 1CIP and 93.3% for 2MET and 2CIP. Between 3 and 30 days, SHAM, 1CIP, and 2CIP produced collagen, while 1MET and 2MET were unchanged. At 30 days, the collagen fiber means percentages for SHAM, PDS, 1MET, 1CIP, 2MET, and 2CIP were 63.7, 60.7, 56.6, 62.6, 51.8, and 61.7, respectively. Antibiotic-eluting matrices showed similar or better oxidative behavior when compared to PDS, except for CIP-eluting matrices, which showed higher levels of PC compared to SHAM or PDS at 30 days. Collectively, our findings indicate that antibiotic-eluting matrices may be an attractive biocompatible drug delivery system to fight periodontopathogens. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B, 2019.
这项研究评估了可降解聚二氧环己酮（PDS）载药电纺系统（以下简称基质）的生物相容性，这些系统中载有甲硝唑（MET）或环丙沙星（CIP），并在大鼠中进行了皮下植入。将 60 只成年雄性大鼠随机分为 6 组：SHAM（假手术）；PDS（不含抗生素的基质）；1MET（一个含 25wt%MET 的基质）；1CIP（一个含 25wt%CIP 的基质）；2MET（两个含 25wt%MET 的基质）；2CIP（两个含 25wt%CIP 的基质）。在第 3 天和第 30 天，对动物的炎症细胞反应（ICR）、胶原蛋白纤维降解和氧化谱（活性氧[ROS]；脂质过氧化[LP]；和蛋白羰基[PC]）进行评估。第 3 天，SHAM、PDS、1MET、1CIP、2MET 和 2CIP 组的离散 ICR 百分比分别为 100%、93.3%、86.7%、76.7%、50%和 66.6%。第 30 天，SHAM、PDS、1MET 和 1CIP 组的离散 ICR 百分比为 100%，2MET 和 2CIP 组为 93.3%。在第 3 天至第 30 天之间，SHAM、1CIP 和 2CIP 产生胶原蛋白，而 1MET 和 2MET 没有变化。第 30 天，SHAM、PDS、1MET、1CIP、2MET 和 2CIP 组的胶原纤维平均百分比分别为 63.7%、60.7%、56.6%、62.6%、51.8%和 61.7%。与 PDS 相比，载抗生素的基质显示出相似或更好的氧化行为，除了载 CIP 的基质，与 SHAM 或 PDS 相比，在第 30 天显示出更高的 PC 水平。总的来说，我们的研究结果表明，载抗生素的基质可能是一种有吸引力的生物相容性药物输送系统，可用于对抗牙周病原体。
