基于硅酸盐纤维的 3D 细胞培养系统用于抗癌药物筛选。
Silicate fiber-based 3D cell culture system for anticancer drug screening.
机构信息
Japan Bio Products Co., Ltd., Aikawa, Kurume, Fukuoka; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Japan.
出版信息
Anticancer Res. 2013 Dec;33(12):5301-9.
BACKGROUND
Three-dimensional (3D) in vitro cultures can recapitulate the physiological in vivo microenvironment. 3D Modeling techniques have been investigated and applied in anticancer drug screening.
MATERIALS AND METHODS
A silicate fiber scaffold was used for 3D cell cultures, and used to model the efficacy of anticancer drugs, such as mytomicin C and doxorubicin.
RESULTS
A unique 3D structure was observed in 13 human tumor cell lines on scaffold, and these cells exhibited higher drug resistance than cells in two-dimensional (2D) cultures. Furthermore, the production of lactate and expression of the nuclear factor-kappa B (NF-κB)-regulated genes B cell lymphoma-2 (BCL2), cyclooxygenase-2 (COX2), and vascular endothelial growth factor (VEGF) were higher in 3D cultures than in 2D cultures.
CONCLUSION
These findings suggest that a 3D model using a silicate fiber scaffold can mimic features of cancer, and is also a suitable model for the evaluation of anticancer drugs in vitro.
背景
三维（3D）体外培养可以再现生理体内微环境。3D 建模技术已被研究并应用于抗癌药物筛选。
材料与方法
使用硅酸盐纤维支架进行 3D 细胞培养，并用于模拟抗癌药物如丝裂霉素 C 和多柔比星的疗效。
结果
在支架上的 13 个人类肿瘤细胞系中观察到独特的 3D 结构，这些细胞比二维（2D）培养中的细胞表现出更高的耐药性。此外，乳酸的产生和核因子-κB（NF-κB）调节基因 B 细胞淋巴瘤-2（BCL2）、环氧化酶-2（COX2）和血管内皮生长因子（VEGF）的表达在 3D 培养中高于 2D 培养。
结论
这些发现表明，使用硅酸盐纤维支架的 3D 模型可以模拟癌症的特征，也是体外评估抗癌药物的合适模型。
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