Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, The Third Military Medical University, Chongqing, People's Republic of China.
PLoS One. 2013 Dec 4;8(12):e81212. doi: 10.1371/journal.pone.0081212. eCollection 2013.
Staphylococcus aureus is a common commensal organism in humans and a major cause of bacteremia and hospital acquired infection. Because of the spread of strains resistant to antibiotics, these infections are becoming more difficult to treat. Therefore, exploration of anti-staphylococcal vaccines is currently a high priority. Iron surface determinant B (IsdB) is an iron-regulated cell wall-anchored surface protein of S. aureus. Alpha-toxin (Hla) is a secreted cytolytic pore-forming toxin. Previous studies reported that immunization with IsdB or Hla protected animals against S. aureus infection. To develop a broadly protective vaccine, we constructed chimeric vaccines based on IsdB and Hla. Immunization with the chimeric bivalent vaccine induced strong antibody and T cell responses. When the protective efficacy of the chimeric bivalent vaccine was compared to that of individual proteins in a murine model of systemic S. aureus infection, the bivalent vaccine showed a stronger protective immune response than the individual proteins (IsdB or Hla). Based on the results presented here, the chimeric bivalent vaccine affords higher levels of protection against S. aureus and has potential as a more effective candidate vaccine.
金黄色葡萄球菌是人类共生的常见病原体，也是菌血症和医院获得性感染的主要原因。由于对抗生素耐药菌株的传播，这些感染越来越难以治疗。因此，探索抗葡萄球菌疫苗目前是当务之急。铁表面决定因子 B（IsdB）是金黄色葡萄球菌中一种受铁调控的细胞表面锚定蛋白。α-毒素（Hla）是一种分泌的细胞溶解孔形成毒素。先前的研究报道，用 IsdB 或 Hla 免疫动物可以预防金黄色葡萄球菌感染。为了开发一种广泛保护的疫苗，我们基于 IsdB 和 Hla 构建了嵌合疫苗。用嵌合二价疫苗免疫可诱导强烈的抗体和 T 细胞反应。当在金黄色葡萄球菌全身感染的小鼠模型中比较嵌合二价疫苗与单一蛋白的保护效力时，二价疫苗显示出比单一蛋白（IsdB 或 Hla）更强的保护免疫应答。基于这里呈现的结果，嵌合二价疫苗提供了更高水平的金黄色葡萄球菌保护，并且作为更有效的候选疫苗具有潜力。
