Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China ; Graduate School of the Chinese Academy of Sciences, Beijing, China.
PLoS One. 2013 Dec 6;8(12):e81377. doi: 10.1371/journal.pone.0081377. eCollection 2013.
Main indoor allergens for humans are from house dust mites. There are more than 30 allergens in Dermatophagoides farinae but only fourteen allergens have been identified from this mite including Der f 1-3, 6, 7, 10, 11, 13-18, and 22. A native allergen protein (Der f 24, 90 kDa) was purified from D. farinae by gel filtration and anionic exchange liquid chromatography combined with IgE immunodetection. Its primary structure was determined by Edman degradation, mass spectrometry analysis and cDNA cloning. Enzyme-linked immunosorbent assay inhibition tests (ELISA-IT), immunoblots, basophil activation test (BAT) and skin prick test (SPT) were performed to evaluate the allergenicity. It was identified as an alpha (α)-actinin containing a CaM-like domain with EF-hand motifs. Der f 24 reacted to sera from 85.4% (35/41) of patients on western blot analysis. It reduced ∼20% sera IgE reactivity to D. farinae extracts on a competitive ELISA. Eighty percent (8/10) of patients with D. farinae allergy showed positive reactions to Der f 24 in skin prick test. The expression of CD63 on basophils from patients was up-regulated by Der f 24 by ∼5.4-fold. Alpha-actinin was identified as a new type of house dust mite allergen. To the best of our knowledge, this is the first report of α-actinin as an allergen.
主要的室内过敏原是尘螨。粉尘螨中有超过 30 种过敏原，但只有 14 种过敏原已被鉴定出来，包括 Der f 1-3、6、7、10、11、13-18 和 22。一种来自粉尘螨的天然过敏原蛋白（Der f 24，90 kDa）通过凝胶过滤和阴离子交换液相色谱结合 IgE 免疫检测法被纯化。它的一级结构通过 Edman 降解、质谱分析和 cDNA 克隆来确定。酶联免疫吸附试验抑制试验（ELISA-IT）、免疫印迹、嗜碱性粒细胞激活试验（BAT）和皮肤点刺试验（SPT）被用来评估其过敏原性。它被鉴定为一种含有 CaM 样结构域的α-肌动蛋白，具有 EF 手基序。Der f 24 在 Western blot 分析中与 85.4%（35/41）的患者血清发生反应。它在竞争性 ELISA 中降低了约 20%血清对粉尘螨提取物的 IgE 反应性。80%（8/10）的粉尘螨过敏患者在皮肤点刺试验中对 Der f 24 呈阳性反应。Der f 24 可上调患者嗜碱性粒细胞 CD63 的表达约 5.4 倍。肌动蛋白被鉴定为一种新型的尘螨过敏原。据我们所知，这是肌动蛋白首次被报道为过敏原。
