Jiang Congli, Fan Xiaoqin, Li Meng, Xing Peng, Liu Xiaoyu, Wu Yulan, Zhang Min, Yang Pingchang, Liu Zhigang
Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. R. China.
Shenzhen Longgang Central Hospital, ENT Institute Shenzhen, Guangdong, P. R. China.
Am J Transl Res. 2015 Jul 15;7(7):1303-13. eCollection 2015.
More than 30 allergens have been identified from Dermatophagoides farina (D. farina), which is one of the main species of house dust mites. The mite allergens are an important factor contributing to allergic disease in the world. As the detection and identification of new allergens is critical for the diagnosis or treatment of allergic diseases, we sought to characterize the profilin of D. farina (Der f 29) in this study. The results showed that 21% of allergic patients displayed positive results in skin prick test with recombinant Der f 29 (rDer f 29) as the specific allergen; specific IgE reactivity to rDer f 29 was shown by Western Blot and ELISA. In addition, rDer f 29 induced bone marrow-derived dendritic cells (DC) to produce T cells immunoglobulin domain and mucin domain protein 4 (TIM4). Moreover, an allergic asthma mouse model was established by challenging with rDer f 29. Airway hyperresponsiveness, serum specific IgE, IgG1, eosinophil infiltration in the allergic mice bronchoalveolar lavage fluid, the cytokines interleukin-4 (IL-4) and interferon-γ (INF-γ) from spleen cells were markedly increased; the histology showed severe inflammation in the lung. In conclusion, Der f 29 is identified as a new type of the house dust mite allergen.
已从粉尘螨（屋尘螨的主要种类之一）中鉴定出30多种过敏原。螨类过敏原是导致全球过敏性疾病的一个重要因素。由于新过敏原的检测和鉴定对过敏性疾病的诊断或治疗至关重要，因此我们在本研究中试图对粉尘螨的肌动蛋白结合蛋白（Der f 29）进行特性分析。结果显示，21%的过敏患者在以重组Der f 29（rDer f 29）作为特异性过敏原的皮肤点刺试验中呈阳性结果；蛋白质印迹法和酶联免疫吸附测定法显示对rDer f 29具有特异性IgE反应性。此外，rDer f 29诱导骨髓来源的树突状细胞（DC）产生T细胞免疫球蛋白结构域和粘蛋白结构域蛋白4（TIM4）。而且，通过用rDer f 29激发建立了过敏性哮喘小鼠模型。过敏性小鼠支气管肺泡灌洗液中的气道高反应性、血清特异性IgE、IgG1、嗜酸性粒细胞浸润以及脾细胞中的细胞因子白细胞介素-4（IL-4）和干扰素-γ（INF-γ）均显著增加；组织学检查显示肺部有严重炎症。总之，Der f 29被鉴定为一种新型的屋尘螨过敏原。
