Lin Jianli, Li Meng, Liu Yulin, Jiang Congli, Wu Yulan, Wang Yuanyuan, Gao Anjian, Liu Zhigang, Yang Pingchang, Liu Xiaoyu
Institute of Allergy & Immunology, Shenzhen University Shenzhen, 518060, China.
The Basic Medical College, Nanchang University Nanchang, 330006, China.
Am J Transl Res. 2015 Jul 15;7(7):1260-70. eCollection 2015.
The house dust mite (HDM), Dermatophagoidesfarinae (D. farina), is one of the most important indoor allergen sources and a major elicitor of allergic asthma; itscharacterization is important in the diagnosis and immunotherapy of mite allergen-relevant diseases. This study aims to characterize a novel allergen, the D. farinae-derived serpin (Der f 27). In this study, the total RNA of D. farinae was extracted, and the Der f 27 gene was cloned and expressed. The allergenicity of recombinant Der f 27 protein was determined by enzyme-linked immunosorbent assay, and Western-blotting with the sera of asthma patients, and skin prick test (SPT) in allergic human subjects. A r-Der f 27 allergic asthma mouse model was established. The cloned Der f 27 gene has been presented at the Gene Bank with an accession number of KM009995. The IgE levels of r-Der f 27 in the serum from r-Der f 27 SPT positive allergic patients were 3 folds more than healthy subjects. The Der f 27 SPT positive ratewas 42.1% in 19 DM-SPT positive patients. Airway hyperresponsiveness, serum specific IgE, and levels of interleukin-4 in the spleen cell culture supernatant were significantly increased in allergic asthma mice sensitized to r-Der f 27. In conclusion, Der f 27 is a new subtype of house mite allergen.
屋尘螨（HDM），即粉尘螨（D. farina），是最重要的室内过敏原来源之一，也是过敏性哮喘的主要诱发因素；其特性在螨过敏原相关疾病的诊断和免疫治疗中具有重要意义。本研究旨在鉴定一种新型过敏原，即粉尘螨衍生的丝氨酸蛋白酶抑制剂（Der f 27）。在本研究中，提取了粉尘螨的总RNA，并克隆和表达了Der f 27基因。通过酶联免疫吸附测定、哮喘患者血清的蛋白质印迹法以及对过敏受试者进行皮肤点刺试验（SPT）来确定重组Der f 27蛋白的致敏性。建立了r-Der f 27过敏性哮喘小鼠模型。克隆的Der f 27基因已提交至基因库，登录号为KM009995。r-Der f 27皮肤点刺试验阳性的过敏患者血清中r-Der f 27的IgE水平比健康受试者高3倍。在19名DM-SPT阳性患者中，Der f 27皮肤点刺试验阳性率为42.1%。对r-Der f 27致敏的过敏性哮喘小鼠的气道高反应性、血清特异性IgE以及脾细胞培养上清液中的白细胞介素-4水平均显著升高。总之，Der f 27是屋螨过敏原的一种新亚型。
