Nettleton G S, Johnson L R, Sehlinger T E
Stain Technol. 1986 Nov;61(6):329-36. doi: 10.3109/10520298609113581.
The tannic acid-phosphomolybdic acid-amido black (TPA) stain has been used primarily for staining hemoglobin. That different dye lots of amido black cause variable staining is documented in the literature. Nine commercial samples of amido black were investigated using thin layer chromatography; all of these dyes contained colored contaminants. Separation of contaminants was achieved using silica gel thin layer chromatography and a solvent system of 95% ethanol:90% phenol:concentrated NH4OH, 12:9:3. TPA staining of red blood cells was improved by using purified amido black.
单宁酸 - 磷钼酸 - 氨基黑（TPA）染色主要用于血红蛋白染色。文献记载不同批次的氨基黑会导致染色效果不同。使用薄层色谱法对9种市售氨基黑样品进行了研究；所有这些染料都含有有色杂质。使用硅胶薄层色谱法和95%乙醇:90%苯酚:浓氨水（12:9:3）的溶剂系统实现了杂质的分离。使用纯化后的氨基黑可改善红细胞的TPA染色效果。
