Hudig Dorothy, Hunter Kenneth W, Diamond W John, Redelman Doug
Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, Nevada, 89557.
Cytometry B Clin Cytom. 2014 Mar;86(2):121-34. doi: 10.1002/cyto.b.21141. Epub 2013 Dec 10.
Human blood monocytes are known to include subsets defined by the expression of CD14 and CD16 but otherwise are often assumed to be relatively homogeneous. However, we had observed additional heterogeneity that led us to a more extensive examination of monocytes.
Blood samples from 200 healthy adults without known immunological abnormalities were examined by analysis with a hematology analyzer and by flow cytometry (FCM) to determine leukocyte differential counts, to identify subsets and to measure expression of monocyte-associated molecules.
The estimated cell counts of monocytes, neutrophils, total lymphocytes, and T cells all varied to a similar extent, that is, ±30-35%. The fractions of monocyte subsets defined by CD14 and CD16 or by CD163 expression also varied among individuals. FCM examinations showed that all the monocyte-associated molecules that were examined varied in expression in this increasing order-CD244, CD4, CD38, CD91, CD11b, toll-like receptor 2 (TLR2), TIA-1, CD14 (on CD14(Br+) cells), CD86, CD80, HLA-DQ, CD33, and HLA-DR.
Human blood monocytes are heterogeneous among healthy adults with respect to cell counts, subsets, and the levels of expression of monocyte-associated molecules. An increase in the "non-classical" (CD14(Lo/Neg) /CD16(+) ) monocyte subset or in the expression of CD11b or TLR2 have known diagnostic/prognostic implications. CD244 and CD4 have well-defined functions on lymphocytes but perform unknown activities on monocytes although their expression appears more narrowly controlled. Together, these data suggest that monocytes should be more extensively examined in both clinical and basic contexts.
已知人类血液单核细胞包含由CD14和CD16表达所定义的亚群，但除此之外通常被认为相对均一。然而，我们观察到了额外的异质性，这促使我们对单核细胞进行更广泛的研究。
对200名无已知免疫异常的健康成年人的血样进行血液学分析仪分析和流式细胞术（FCM）检测，以确定白细胞分类计数、识别亚群并测量单核细胞相关分子的表达。
单核细胞、中性粒细胞、总淋巴细胞和T细胞的估计细胞计数变化程度相似，即±30 - 35%。由CD14和CD16或CD163表达所定义的单核细胞亚群比例在个体间也有所不同。FCM检测显示，所有检测的单核细胞相关分子的表达按以下递增顺序变化：CD244、CD4、CD38、CD91、CD11b、Toll样受体2（TLR2）、TIA - 1、CD14（在CD14(Br+)细胞上）、CD86、CD80、HLA - DQ、CD33和HLA - DR。
在健康成年人中，人类血液单核细胞在细胞计数、亚群以及单核细胞相关分子的表达水平方面存在异质性。“非经典”（CD14(Lo/Neg)/CD16(+)）单核细胞亚群增加或CD11b或TLR2表达增加具有已知的诊断/预后意义。CD244和CD4在淋巴细胞上具有明确的功能，但在单核细胞上发挥未知的作用，尽管它们的表达似乎受到更严格的调控。总之，这些数据表明，在临床和基础研究中都应更广泛地研究单核细胞。
