Department of Morphology, Surgery and Experimental Medicine and LTTA Centre, University of Ferrara, Via Fossato di Mortara 70, 44100 Ferrara, Italy.
Mediators Inflamm. 2013;2013:561689. doi: 10.1155/2013/561689. Epub 2013 Nov 13.
Twenty patients affected by chronic venous disease (CVD) in tertiary venous network and/or saphenous vein were analyzed before surgical ablation by echo-color-doppler for the hemodynamic parameters reflux time (RT) and resistance index (RI), a negative and a positive prognostic factor, respectively. RT and RI were next correlated with relevant in vitro parameters of venous endothelial cells (VEC) obtained from surgical specimens, such as cell migration in response to serum gradient, proliferation index, intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 expression, as well as cytokines release. Of interest, ICAM-1 expression in patient-derived VEC cultures correlated positively with RT and negatively with RI. Moreover, RT showed a positive correlation with the baseline osteoprotegerin (OPG) expression by VEC and an inverse correlation with VEC proliferation index. On the other hand, RI correlated positively with TNF-related apoptosis inducing ligand (TRAIL) expression. Among the cytokines released by VEC, GM-CSF showed a positive correlation with VEC proliferation and TRAIL expression and a negative correlation with OPG, ICAM-1 and VCAM-1 expression. Since in vitro recombinant GM-CSF induced VEC proliferation and counteracted the induction of ICAM-1, VCAM-1 and OPG upon exposure to TNF-α, our data suggest an anti-inflammatory activity of GM-CSF on venous endothelial cells.
20 例慢性静脉疾病（CVD）患者在接受手术消融前通过回声彩色多普勒分析了血流动力学参数反流时间（RT）和阻力指数（RI），这两个参数分别是负向和正向的预后因素。接下来，将 RT 和 RI 与从手术标本中获得的静脉内皮细胞（VEC）的相关体外参数相关联，例如细胞对血清梯度的迁移、增殖指数、细胞间黏附分子（ICAM）-1 和血管细胞黏附分子（VCAM）-1 的表达以及细胞因子的释放。有趣的是，患者来源的 VEC 培养物中的 ICAM-1 表达与 RT 呈正相关，与 RI 呈负相关。此外，RT 与 VEC 基础骨保护素（OPG）表达呈正相关，与 VEC 增殖指数呈负相关。另一方面，RI 与 TNF 相关凋亡诱导配体（TRAIL）表达呈正相关。在 VEC 释放的细胞因子中，GM-CSF 与 VEC 增殖和 TRAIL 表达呈正相关，与 OPG、ICAM-1 和 VCAM-1 表达呈负相关。由于体外重组 GM-CSF 诱导 VEC 增殖，并在 TNF-α作用下抵消了 ICAM-1、VCAM-1 和 OPG 的诱导，我们的数据表明 GM-CSF 对静脉内皮细胞具有抗炎活性。
