Tardy Blaise L, Dam Henk H, Kamphuis Marloes M J, Richardson Joseph J, Caruso Frank
Department of Chemical and Biomolecular Engineering, The University of Melbourne , Victoria 3010, Australia.
Biomacromolecules. 2014 Jan 13;15(1):53-9. doi: 10.1021/bm401244a. Epub 2013 Dec 13.
Thermodynamically assembled core-shell nanocarriers are potential candidates for drug delivery applications due to their submicrometer size and the ability to load drugs into their hydrophobic core. Herein, we describe the formation of core-shell particles that consist of noncovalent polymers, that is, polyrotaxanes (PRXs), that form an α-cyclodextrin (αCD) core surrounded by a corona of low-fouling poly(ethylene glycol) (PEG). The PRX core-shell particles are able to sequester small organic molecules, such as pyrene and calcein, releasing these small molecules during degradation. The small, cellular peptide, glutathione, was used to degrade the particles through the reductive cleavage of disulfide bonds that stabilize the individual PRX polymers. Cleavage of a single bond allows for the degradation of the supramolecular-polymer, making these PRX core-shell particles highly responsive. Furthermore, these particles demonstrate negligible cytotoxicity in mammalian cells, making them promising carriers for future drug delivery research.
由于其亚微米尺寸以及将药物载入疏水核心的能力，热力学组装的核壳纳米载体是药物递送应用的潜在候选者。在此，我们描述了由非共价聚合物即聚轮烷（PRXs）组成的核壳颗粒的形成，这些聚轮烷形成了一个由低污染聚乙二醇（PEG）冠层包围的α-环糊精（αCD）核心。PRX核壳颗粒能够螯合小分子有机化合物，如芘和钙黄绿素，并在降解过程中释放这些小分子。小细胞肽谷胱甘肽通过稳定单个PRX聚合物的二硫键的还原裂解来降解颗粒。单个键的断裂允许超分子聚合物的降解，使这些PRX核壳颗粒具有高度响应性。此外，这些颗粒在哺乳动物细胞中表现出可忽略不计的细胞毒性，使其成为未来药物递送研究的有前途的载体。
