Chen Zhenhong, Liu Mengying, Cui Yujun, Wang Li, Zhang Yiquan, Qiu Jingfu, Yang Ruifu, Liu Changting, Zhou Dongsheng
Nanlou Respiratory Diseases Department, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Future Microbiol. 2014;9(1):21-32. doi: 10.2217/fmb.13.137.
To establish a PCR-based genotyping method for clinical Klebsiella pneumoniae.
MATERIALS & METHODS: The prevalence of six serotype markers, 41 large variably presented gene clusters, and seven additional virulence markers were screened by PCR in 327 clinical K. pneumoniae strains from China.
Detection of serotype markers enabled the identification of capsular serotypes K1, K2, K5, K20, K54 and K57. K. pneumoniae isolates of different origins gave distinct profiles of virulence loci, allowing us to gain a full overview of virulence gene distribution of the strains tested. A novel genotyping scheme was established to group clinical K. pneumoniae strains into distinct complexes based on the profiles of large variably presented gene clusters and virulence markers.
This PCR-based genotyping method would be useful to not only characterize genetic diversity and virulence gene distribution, but also for genotyping, origin tracing and risk estimation of K. pneumoniae.
建立一种基于聚合酶链反应（PCR）的临床肺炎克雷伯菌基因分型方法。
采用PCR技术对来自中国的327株临床肺炎克雷伯菌菌株进行六种血清型标记、41个大的可变呈现基因簇以及另外七种毒力标记的流行情况筛查。
血清型标记检测能够鉴定出K1、K2、K5、K20、K54和K57荚膜血清型。不同来源的肺炎克雷伯菌分离株具有不同的毒力基因座谱，使我们能够全面了解所测试菌株的毒力基因分布情况。基于大的可变呈现基因簇和毒力标记的谱型，建立了一种新的基因分型方案，将临床肺炎克雷伯菌菌株分为不同的复合体。
这种基于PCR的基因分型方法不仅有助于表征遗传多样性和毒力基因分布，还可用于肺炎克雷伯菌的基因分型、溯源和风险评估。
