School of Dentistry, Universidad Santo Tomas, Floridablanca Bucaramanga, Santander, Colombia.
J Endod. 2011 Jul;37(7):934-7. doi: 10.1016/j.joen.2011.03.035. Epub 2011 May 18.
The purpose of this study was to quantify the effect of moderate and severe orthodontic forces on calcitonin gene-related peptide (CGRP) expression in healthy human dental pulp.
Thirty human dental pulp samples were obtained from healthy premolars in which extraction was indicated for orthodontic reasons. Before extraction, teeth were divided into 3 groups of 10 premolars each: (1) the control group: healthy premolars without application of orthodontic forces; (2) the moderate force group: a 56-g force was applied to the premolars for 24 hours; and (3) the severe force group: a 224-g force was applied to the premolars for 24 hours. All dental pulp samples were processed, and CGRP was measured by radioimmunoassay.
Greater CGRP expression was found in the severe force group followed by the moderate force group. The lower CGRP values were for the control group. The Kruskal-Wallis test showed statistically significant differences between groups (P < .0001). Least significant difference (LSD) post hoc tests showed statistically significant differences in CGRP expression between the control group and the severe force group (P < .0001) but not with the moderate force group (P = .06). Differences between the moderate and severe force groups were statistically significant (P < .0001).
CGRP expression in human dental pulp increases when teeth are submitted to severe orthodontic forces.
本研究的目的是定量研究中度和重度正畸力对健康人牙髓降钙素基因相关肽（CGRP）表达的影响。
从因正畸需要而拔除的健康前磨牙中获得 30 个人类牙髓样本。在拔牙前，将牙齿分为 3 组，每组 10 颗前磨牙：（1）对照组：未施加正畸力的健康前磨牙；（2）中度力组：在前磨牙上施加 56g 的力 24 小时；（3）重度力组：在前磨牙上施加 224g 的力 24 小时。所有牙髓样本均经处理，放射免疫法测量 CGRP。
重度力组的 CGRP 表达较高，其次是中度力组。对照组的 CGRP 值较低。Kruskal-Wallis 检验显示组间差异有统计学意义（P<0.0001）。LSD 事后检验显示对照组与重度力组之间 CGRP 表达差异有统计学意义（P<0.0001），但与中度力组无差异（P=0.06）。中度力组与重度力组之间的差异有统计学意义（P<0.0001）。
当牙齿受到重度正畸力时，人牙髓中的 CGRP 表达增加。
