Velaga Manoj Kumar, Basuri Charan Kumar, Robinson Taylor Kendra S, Yallapragada Prabhakara Rao, Rajanna Sharada, Rajanna Bettaiya
Department of Zoology, Andhra University , Visakhapatnam 530003 , India , and.
Drug Chem Toxicol. 2014 Jul;37(3):357-64. doi: 10.3109/01480545.2013.866137. Epub 2013 Dec 12.
Bacopa monniera is a rejuvenating herb for brain cells enhancing learning and cognitive ability. In the present investigation, the ameliorative effects of Bacopa monniera were examined against lead-induced oxidative stress in different regions of rat brain. Male rats were divided into five groups: control (1000 ppm sodium acetate) and exposed (1000 ppm lead acetate) for 4 weeks; DMSA (Meso-2,3-Dimercaptosuccinic acid)-treated (90 mg/kg body weight/day); Bacopa monniera-treated (BM) (10 mg/kg body weight/day) and a combination of BM + DMSA for seven consecutive days after 4 weeks of lead exposure. After treatment, the whole brain was isolated by sacrificing rats and four regions were separated namely cerebellum, hippocampus, frontal cortex and brain stem. Results indicated a significant (p < 0.05) increase in reactive oxygen species (ROS), lipid peroxidation products (LPP) and total protein carbonyl content (TPCC) in association with tissue metal content in all the four regions of brain for exposed group compared with their respective controls. However, the lead-induced ROS, LPP, TPCC and tissue metal content were lowered on treatment with Bacopa monniera, almost reaching the control group values in all the above brain regions compared to DMSA and a combination therapy. Results suggest that Bacopa monniera can mitigate the lead induced-oxidative stress tissue specifically by pharmacologic interventions which encompass both chelation as well as antioxidant functions.
假马齿苋是一种能使脑细胞恢复活力、增强学习和认知能力的草药。在本研究中，研究了假马齿苋对铅诱导的大鼠脑不同区域氧化应激的改善作用。将雄性大鼠分为五组：对照组（1000 ppm醋酸钠）和暴露组（1000 ppm醋酸铅），暴露4周；二巯基丁二酸（DMSA）治疗组（90 mg/kg体重/天）；假马齿苋治疗组（BM）（10 mg/kg体重/天），以及在铅暴露4周后连续7天给予BM + DMSA联合治疗。治疗后，处死大鼠分离出全脑，并分离出四个区域，即小脑、海马体、额叶皮质和脑干。结果表明，与各自的对照组相比，暴露组大脑所有四个区域的活性氧（ROS）、脂质过氧化产物（LPP）和总蛋白羰基含量（TPCC）均显著（p < 0.05）增加，且与组织金属含量相关。然而，与DMSA和联合治疗相比，用假马齿苋治疗可降低铅诱导的ROS、LPP、TPCC和组织金属含量，在上述所有脑区几乎达到对照组水平。结果表明，假马齿苋可以通过包括螯合和抗氧化功能的药理干预，特异性地减轻铅诱导的氧化应激组织损伤。
