Department of Biochemistry and Nutrition, Central Food Technological Research Institute (Council of Scientific and Industrial Research), Mysore 570020, Karnataka, India.
Phytomedicine. 2011 Feb 15;18(4):317-26. doi: 10.1016/j.phymed.2010.08.005. Epub 2010 Sep 18.
Bacopa monnieri (BM) an herb, found throughout the Indian subcontinent in wet, damp and marshy areas is used in Ayurvedic system of medicine for improving intellect/memory, treatment of anxiety and neuropharmacological disorders. Although extensively given to children as a memory enhancer, no data exists on its ability to modulate neuronal oxidative stress in prepubertal animal models. Hence in this study, we examined if dietary intake of BM leaf powder has the propensity to modulate endogenous markers of oxidative stress, redox status (reduced GSH, thiol status), response of antioxidant defenses (enzymic), protein oxidation and cholinergic function in various brain regions of prepubertal (PP) mice. PP mice maintained on a BM-enriched diet (0.5 and 1%) for 4 weeks showed a significant diminution of basal oxidative markers (malondialdehyde levels, reactive species generation, hydroperoxide levels and protein carbonyls) in both cytoplasm and mitochondria of all brain regions. This was accompanied with enhanced reduced glutathione, thiol levels and elevated activities of antioxidant enzymes (catalase, glutathione peroxidase, superoxide dismutase). Significant reduction in the activity of acetyl cholinesterase enzyme in all brain regions suggested the potential of BM leaf powder to modulate cholinergic function. Further evidence that dietary intake of BM leaf powder confers the prepubertal brain with additional capacity to cope up with neurotoxic prooxidants was obtained by exposing cortical/cerebellar synaptosomes of normal and BM fed mice to 3-nitropropionic acid (3-NPA). While synaptosomes from control mice exhibited a concentration related lipid peroxidation and ROS generation, synaptosomes obtained from BM fed mice showed only a marginal induction at the highest concentration clearly suggesting their increased resistance to 3-NPA-induced oxidative stress. Collectively these data clearly indicate the potential of Bacopa monnieri to modulate endogenous markers of oxidative stress in brain tissue of PP mice. Based on these results, it is hypothesized that dietary intake of BM leaf powder confers neuroprotective advantage and is likely to be effective as a prophylactic/therapeutic agent for neurodegenerative disorders involving oxidative stress.
印度产益智草（Bacopa monnieri，简称 BM）是一种草本植物，广泛分布于印度次大陆的湿地、沼泽等潮湿地区。在印度传统医学阿育吠陀体系中，它被用于提高智力/记忆力、治疗焦虑症和神经药理学障碍。虽然这种草药被广泛用于儿童提高记忆力，但目前还没有数据表明它能够调节未成年动物模型的神经元氧化应激。因此，在这项研究中，我们研究了益智草叶粉的饮食摄入是否具有调节未成年（prepubertal，简称 PP）小鼠大脑各区域内氧化应激内源性标志物、氧化还原状态（还原型谷胱甘肽、巯基状态）、抗氧化防御反应（酶）、蛋白质氧化和胆碱能功能的倾向。4 周内，PP 小鼠维持在富含益智草的饮食（0.5%和 1%）中，结果显示，所有大脑区域的细胞质和线粒体中的基础氧化标志物（丙二醛水平、活性物质生成、过氧化物水平和蛋白质羰基）显著减少。这伴随着还原型谷胱甘肽、巯基水平的提高和抗氧化酶（过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶）活性的升高。所有大脑区域中的乙酰胆碱酯酶活性显著降低表明，益智草叶粉具有调节胆碱能功能的潜力。进一步的证据表明，通过将正常和 BM 喂养的小鼠的皮质/小脑突触体暴露于 3-硝基丙酸（3-NPA）来摄入 BM 叶粉会使未成年大脑获得额外的应对神经毒性促氧化剂的能力。当控制组的突触体显示出与浓度相关的脂质过氧化和 ROS 生成时，来自 BM 喂养组的突触体仅在最高浓度下显示出轻微的诱导，这清楚地表明它们对 3-NPA 诱导的氧化应激具有更高的抵抗力。总的来说，这些数据清楚地表明益智草具有调节未成年小鼠大脑组织内氧化应激内源性标志物的潜力。基于这些结果，我们假设摄入益智草叶粉可以赋予神经保护优势，并可能作为涉及氧化应激的神经退行性疾病的预防/治疗剂有效。
Methods Mol Biol. 2024
Curr Neuropharmacol. 2021
Zotero 插件