Di Marzo V, Tippins J R, Morris H R
Biochem Int. 1986 Dec;13(6):933-42.
The effect of four neuropeptides and acetylcholine on the release of leukotrienes LTC4, LTD4 and LTE4 from platelet activating factor-stimulated rat lung and ionophore A23187-stimulated guinea pig lung, as detected by the combined use of HPLC and radioimmunoassay, was studied. Both vasoactive intestinal peptide and calcitonin gene-related peptide were found to inhibit the release of leukotrienes in both preparations. This effect was most marked in platelet activating factor-stimulated rat lung, where inhibition of LTC4 release was more pronounced than either inhibition of LTD4 or LTE4 production. The effect of vasoactive intestinal peptide on LTC4 biosynthesis was dose-related in rat lung. Neither substance P nor beta-endorphin were found to inhibit leukotriene release in rat lung. Vasoactive intestinal peptide inhibition of leukotriene release is independent from its actions on the muscarinic receptor, since acetylcholine was found to have no effect in the same preparation.
通过高效液相色谱（HPLC）和放射免疫测定法联合检测，研究了四种神经肽和乙酰胆碱对血小板活化因子刺激的大鼠肺组织以及离子载体A23187刺激的豚鼠肺组织中白三烯LTC4、LTD4和LTE4释放的影响。发现血管活性肠肽和降钙素基因相关肽均可抑制两种组织中白三烯的释放。这种作用在血小板活化因子刺激的大鼠肺组织中最为明显，其中对LTC4释放的抑制比对LTD4或LTE4生成的抑制更为显著。血管活性肠肽对大鼠肺组织中LTC4生物合成的作用具有剂量相关性。在大鼠肺组织中，未发现P物质或β-内啡肽可抑制白三烯的释放。血管活性肠肽对白三烯释放的抑制作用与其对毒蕈碱受体的作用无关，因为在相同的组织中发现乙酰胆碱无作用。
