静息膜的钾通透性是否受兴奋性钾通道的控制？
Is the K permeability of the resting membrane controlled by the excitable K channel?
作者信息
Chang D C
出版信息
Biophys J. 1986 Dec;50(6):1095-100. doi: 10.1016/S0006-3495(86)83553-6.
Abstract
To test whether or not the potassium permeability of the resting membrane is controlled by the excitable K channels (delayed rectifier), we examined changes in the Na and K permeability ratio, PNa/PK, of the squid axon before and after the excitable K channels were blocked. The blockage of the K channels was accomplished by three independent methods: internal application of tetraethylammonium, internal application of 4-aminopyridine plus Cs, and prolong internal perfusion of NaF solution. The permeability ratio was determined using two different methods: the conventional electrophysiological method and a new method based on the measurements of the hyperpolarizing effect of Na removal. We found that blocking the K channels did not cause a proportional decrease in the K permeability of the resting membrane, suggesting that the semipermeable property of the resting membrane is not determined by the excitable K channels.
摘要
为了测试静息膜的钾通透性是否受可兴奋钾通道（延迟整流器）控制，我们检测了乌贼轴突在可兴奋钾通道被阻断前后钠钾通透性比值（PNa/PK）的变化。钾通道的阻断通过三种独立方法实现：内部施加四乙铵、内部施加4-氨基吡啶加铯，以及延长NaF溶液的内部灌注时间。通透性比值通过两种不同方法测定：传统电生理方法和基于测量钠去除的超极化效应的新方法。我们发现阻断钾通道并未导致静息膜钾通透性成比例下降，这表明静息膜的半透性并非由可兴奋钾通道决定。
相似文献
1
Is the K permeability of the resting membrane controlled by the excitable K channel?静息膜的钾通透性是否受兴奋性钾通道的控制？
Biophys J. 1986 Dec;50(6):1095-100. doi: 10.1016/S0006-3495(86)83553-6.
2
A comparative study of the effects of tetrodotoxin and the removal of external Na+ on the resting potential: evidence of separate pathways for the resting and excitable Na currents in squid axon.河豚毒素和去除细胞外钠离子对静息电位影响的比较研究：乌贼轴突中静息钠电流和可兴奋钠电流存在独立通道的证据
Cell Mol Neurobiol. 1985 Dec;5(4):311-20. doi: 10.1007/BF00755398.
3
Sodium channel permeation in squid axons. I: Reversal potential experiments.乌贼轴突中的钠通道通透。I：反转电位实验。
J Physiol. 1980 Oct;307:217-42. doi: 10.1113/jphysiol.1980.sp013432.
4
Ionic permeability of K, Na, and Cl in potassium-depolarized nerve. Dependency on pH, cooperative effects, and action of tetrodotoxin.钾离子去极化神经中钾、钠和氯的离子通透性。对pH的依赖性、协同效应及河豚毒素的作用。
Biophys J. 1981 Sep;35(3):677-97. doi: 10.1016/S0006-3495(81)84820-5.
5
Modulation of aminopyridine block of potassium currents in squid axon.乌贼轴突中钾电流的氨基吡啶阻断调节
Biophys J. 1986 Oct;50(4):637-44. doi: 10.1016/S0006-3495(86)83503-2.
6
Chemically induced K+ conduction noise in squid axon.化学诱导的乌贼轴突钾离子传导噪声
J Membr Biol. 1979 May 21;47(2):99-112. doi: 10.1007/BF01876111.
7
Sodium ions as blocking agents and charge carriers in the potassium channel of the squid giant axon.钠离子作为乌贼巨大轴突钾通道中的阻断剂和电荷载体。
J Gen Physiol. 1977 Dec;70(6):707-24. doi: 10.1085/jgp.70.6.707.
8
Cation permeability ratios of sodium channels in normal and grayanotoxin-treated squid axon membranes.正常和经灰藓毒素处理的鱿鱼轴突膜中钠通道的阳离子通透率比值。
J Membr Biol. 1977 Mar 23;31(4):359-81. doi: 10.1007/BF01869413.
9
[Physiology and pharmacology of the Na and K channels of axon membranes].[轴突膜钠通道和钾通道的生理学与药理学]
J Physiol (Paris). 1985;80(2):120-8.
10
Single potassium channels with delayed rectifier behavior from lobster axon membranes.来自龙虾轴突膜的具有延迟整流行为的单个钾通道。
Biophys J. 1984 Jan;45(1):289-99. doi: 10.1016/S0006-3495(84)84155-7.
引用本文的文献
1
Not so optimal: The evolution of mutual information in potassium voltage-gated channels.并非最优：钾电压门控通道中互信息的进化。
PLoS One. 2023 Feb 3;18(2):e0264424. doi: 10.1371/journal.pone.0264424. eCollection 2023.
2
Is the delayed rectifier the major pathway for resting k current?延迟整流器是静息钾电流的主要途径吗？
Biophys J. 1988 Nov;54(5):971-2. doi: 10.1016/S0006-3495(88)83034-0.
3
Opening and closing of KCNKO potassium leak channels is tightly regulated.KCNKO钾离子渗漏通道的开启和关闭受到严格调控。
J Gen Physiol. 2000 Nov;116(5):721-34. doi: 10.1085/jgp.116.5.721.
4
Cell-attached measurements of the firing threshold of rat hippocampal neurones.大鼠海马神经元放电阈值的细胞贴附式测量
J Physiol. 1999 Jun 15;517 ( Pt 3)(Pt 3):791-804. doi: 10.1111/j.1469-7793.1999.0791s.x.
5
On the relationship between resting potential and the delayed rectifier in squid axons.关于鱿鱼轴突静息电位与延迟整流器之间的关系。
Biophys J. 1988 Nov;54(5):969-70. doi: 10.1016/S0006-3495(88)83033-9.
6
The potassium conductance of the resting squid axon and its blockage by clinical concentrations of general anaesthetics.静息乌贼轴突的钾离子电导及其被临床浓度全身麻醉剂的阻断作用。
J Physiol. 1988 Aug;402:363-74. doi: 10.1113/jphysiol.1988.sp017209.
7
Theoretical studies of impulse propagation in serotonergic axons.血清素能轴突中冲动传播的理论研究。
Biol Cybern. 1992;66(5):399-406. doi: 10.1007/BF00197719.
8
A TEA-insensitive flickering potassium channel active around the resting potential in myelinated nerve.一种对茶不敏感的闪烁钾通道，在有髓神经的静息电位附近活跃。
J Membr Biol. 1992 Nov;130(2):149-62. doi: 10.1007/BF00231893.
本文引用的文献
1
Single Ca Dependent K Currents in HeLa Cancer Cells.人宫颈癌HeLa细胞中的单钙依赖性钾电流
Biophys J. 1984 Jan;45(1):66-8. doi: 10.1016/S0006-3495(84)84111-9.
2
The effect of sodium ions on the electrical activity of giant axon of the squid.钠离子对鱿鱼巨大轴突电活动的影响。
J Physiol. 1949 Mar 1;108(1):37-77. doi: 10.1113/jphysiol.1949.sp004310.
3
The components of membrane conductance in the giant axon of Loligo.枪乌贼巨大轴突膜电导的组成成分。
J Physiol. 1952 Apr;116(4):473-96. doi: 10.1113/jphysiol.1952.sp004718.
4
ANOMALOUS RECTIFICATION IN THE SQUID GIANT AXON INJECTED WITH TETRAETHYLAMMONIUM CHLORIDE.注射氯化四乙铵的乌贼巨轴突中的反常整流
J Gen Physiol. 1965 May;48(5):859-72. doi: 10.1085/jgp.48.5.859.
5
THE EFFECT OF DILUTING THE INTERNAL SOLUTION ON THE ELECTRICAL PROPERTIES OF A PERFUSED GIANT AXON.稀释内部溶液对灌流巨型轴突电特性的影响。
J Physiol. 1964 Apr;170(3):541-60. doi: 10.1113/jphysiol.1964.sp007348.
6
The effects of changes in internal ionic concentrations on the electrical properties of perfused giant axons.内部离子浓度变化对灌流巨型轴突电特性的影响。
J Physiol. 1962 Nov;164(2):355-74. doi: 10.1113/jphysiol.1962.sp007026.
7
Ionic movements and electrical activity in giant nerve fibres.巨神经纤维中的离子运动与电活动。
Proc R Soc Lond B Biol Sci. 1958 Jan 1;148(930):1-37. doi: 10.1098/rspb.1958.0001.
8
Demonstration of two stable potential states in the squid giant axon under tetraethylammonium chloride.在氯化四乙铵作用下乌贼巨大轴突中两种稳定电位状态的证明。
J Gen Physiol. 1957 Jul 20;40(6):859-85. doi: 10.1085/jgp.40.6.859.
9
A quantitative description of membrane current and its application to conduction and excitation in nerve.膜电流的定量描述及其在神经传导和兴奋中的应用。
J Physiol. 1952 Aug;117(4):500-44. doi: 10.1113/jphysiol.1952.sp004764.
10
Survival of K+ permeability and gating currents in squid axons perfused with K+-free media.在无钾培养基灌注的鱿鱼轴突中钾离子通透性和门控电流的存活情况。
J Gen Physiol. 1980 Jan;75(1):61-78. doi: 10.1085/jgp.75.1.61.
Zotero 插件