来自龙虾轴突膜的具有延迟整流行为的单个钾通道。
Single potassium channels with delayed rectifier behavior from lobster axon membranes.
作者信息
Coronado R, Latorre R, Mautner H G
出版信息
Biophys J. 1984 Jan;45(1):289-99. doi: 10.1016/S0006-3495(84)84155-7.
Abstract
Single-channel potassium currents from lobster axon membranes were studied in planar bilayers made from monolayers. Channel-opening events are grouped by time, forming bursts with an average duration of 4.5 ms. The mean open time at 0 mV is 1.8 ms. The frequency of bursts is voltage dependent, increasing e-fold per 12-16 mV. At sufficiently high positive voltages, channels inactivate. Measured from reversal potentials, channels discriminate against Na+ by a permeability ratio PNa/PK of 1:30. The channel is blocked by tetraethylammonium and nonyltrimethylammonium in a voltage-dependent manner and at concentrations similar to those used in whole-axon experiments. Voltage-dependent block by Cs+ suggests that more than one ion may occupy the channel simultaneously. The kinetics and selectivity of this channel suggest that purified axolemma contains active K+ channels that are likely to participate in delayed rectification in the lobster axon membrane.
摘要
在由单层制成的平面双层膜中研究了龙虾轴突膜的单通道钾电流。通道开放事件按时间分组，形成平均持续时间为4.5毫秒的爆发。在0 mV时的平均开放时间为1.8毫秒。爆发频率与电压有关，每12 - 16 mV增加一倍。在足够高的正电压下，通道会失活。从反转电位测量，通道对Na+的通透性比PNa/PK为1:30，从而对Na+具有选择性。该通道被四乙铵和壬基三甲基铵以电压依赖性方式阻断，且阻断浓度与全轴突实验中使用的浓度相似。Cs+的电压依赖性阻断表明可能有不止一个离子同时占据通道。该通道的动力学和选择性表明，纯化的轴膜含有可能参与龙虾轴突膜延迟整流的活性钾通道。
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