Sanitá Paula Volpato, Zago Chaiene Evelin, Pavarina Ana Cláudia, Jorge Janaina Habib, Machado Ana Lúcia, Vergani Carlos Eduardo
Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista. Rua Humaitá, Araraquara, Sao Paulo, Brazil.
Mycoses. 2014 Jun;57(6):351-7. doi: 10.1111/myc.12162. Epub 2013 Dec 13.
The secretion of hydrolytic enzymes is a fundamental virulence factor of Candida albicans to develop disease. The objective of this study was to characterise the virulence of 148 clinical isolates of C. albicans from oral candidiasis by assessing the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP). Isolates were obtained from healthy subjects (HS) and diabetics (DOC) and non-diabetics with oral candidiasis (NDOC). An aliquot (5 μl) of each cell suspension was inoculated on PL and SAP agar plates and incubated. Enzymes secretion was detected by the formation of an opaque halo around the colonies and enzymatic activity (PZ) was determined by the ratio between colony diameter and colony diameter plus the halo zone. Statistical comparisons were made by a one-way anova followed by Tukey's post hoc test (α = 0.05). The clinical sources of C. albicans had significant effect (P < 0.001) on the PZ values of both enzymes. For PL, clinical isolates from NDOC and DOC had highest enzymatic activity than those from HS (P < 0.05), with no significant differences between them (P = 0.506). For SAP, C. albicans from NDOC showed the lower enzymatic activity (P < 0.001). There were no significant differences between isolates from HS and DOC (P = 0.7051). C. albicans isolates from NDOC and DOC patients showed an increased production of PL.
水解酶的分泌是白色念珠菌引发疾病的一种基本毒力因子。本研究的目的是通过评估磷脂酶（PL）和分泌天冬氨酸蛋白酶（SAP）的表达来表征148株来自口腔念珠菌病的白色念珠菌临床分离株的毒力。分离株取自健康受试者（HS）、糖尿病患者（DOC）以及患有口腔念珠菌病的非糖尿病患者（NDOC）。将每份细胞悬液的一份等分试样（5μl）接种到PL和SAP琼脂平板上并进行培养。通过菌落周围形成不透明晕圈来检测酶的分泌，并通过菌落直径与菌落直径加晕圈区域的比值来确定酶活性（PZ）。采用单因素方差分析并随后进行Tukey事后检验（α = 0.05）进行统计学比较。白色念珠菌的临床来源对两种酶的PZ值均有显著影响（P < 0.001）。对于PL，来自NDOC和DOC的临床分离株的酶活性高于来自HS的分离株（P < 0.05），它们之间无显著差异（P = 0.506）。对于SAP，来自NDOC的白色念珠菌显示出较低的酶活性（P < 0.001）。来自HS和DOC的分离株之间无显著差异（P = 0.7051）。来自NDOC和DOC患者的白色念珠菌分离株显示出PL产量增加。
