Kumar C P Girish, Kumar S Suresh Jothi, Menon Thangam
Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, 600 113, India.
Mycopathologia. 2006 Apr;161(4):213-8. doi: 10.1007/s11046-005-0157-4.
Sixty-one isolates of Candida recovered from HIV seropositive and cancer patients were studied for elaboration of putative virulence determinants--phospholipase (PL) and secreted aspartyl proteinase (Sap). Forty two (68.85%) isolates examined were PL producers and 51 (83.6%) were positive for Sap. 57.37% (35/61) isolates produced both enzymes. Enzymatic activity was more pronounced in Candida albicans with 100% PL and 94.1% Sap activity. In contrast, non-C. albicans species demonstrated only 29.6% PL and 70.3% Sap activity, indicating interplay of other virulence determinants in these yeasts in colonization and disease.
对从HIV血清阳性患者和癌症患者中分离出的61株念珠菌进行研究，以确定其假定的毒力决定因素——磷脂酶（PL）和分泌天冬氨酸蛋白酶（Sap）。检测的61株菌株中，42株（68.85%）产生PL，51株（83.6%）Sap检测呈阳性。57.37%（35/61）的菌株同时产生这两种酶。白色念珠菌的酶活性更为显著，PL活性为100%，Sap活性为94.1%。相比之下，非白色念珠菌的PL活性仅为29.6%，Sap活性为70.3%，这表明这些酵母菌中的其他毒力决定因素在定植和疾病过程中相互作用。
