Sanitá Paula Volpato, Zago Chaiene Evelin, Mima Ewerton Garcia de Oliveira, Pavarina Ana Cláudia, Jorge Janaina Habib, Machado Ana Lúcia, Vergani Carlos Eduardo
Substitute Professor, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
PhD Student, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Jul;118(1):84-91. doi: 10.1016/j.oooo.2014.03.020. Epub 2014 Apr 8.
To evaluate the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP) by Candida glabrata and C tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates).
After incubation, the supernatants and pellets of the isolates were used for the enzymatic assays and quantification of colony-forming units (CFU), respectively. Colorimetric tests were used with phosphatidylcholine as a substrate for PL and azocasein as a substrate for SAP, and the absorbances of the samples were measured. Enzymatic rates were calculated, and values were normalized by CFU. Results were analyzed with factorial analyses of variance (α = .05).
C tropicalis and C glabrata were proteolytic and phospholipolytic. The clinical sources of isolates had no significant effect on the enzymatic activities (P > .05). C tropicalis had significantly higher enzymatic activity for both PL and SAP (P < .001) than did C glabrata.
C tropicalis isolates produced significantly higher amounts of both enzymes than did the C glabrata isolates.
评估从健康参与者（16株分离株）、糖尿病合并口腔念珠菌病患者（10株分离株）和非糖尿病口腔念珠菌病患者（25株分离株）的义齿生物膜中获得的光滑念珠菌和热带念珠菌的磷脂酶（PL）和分泌天冬氨酸蛋白酶（SAP）的表达。
培养后，分离株的上清液和菌 pellet 分别用于酶活性测定和菌落形成单位（CFU）定量。以磷脂酰胆碱为PL底物、偶氮酪蛋白为SAP底物进行比色试验，并测量样品的吸光度。计算酶活性速率，并通过CFU对数值进行标准化。结果采用方差分析（α = 0.05）进行分析。
热带念珠菌和光滑念珠菌具有蛋白水解和磷脂水解作用。分离株的临床来源对酶活性无显著影响（P > 0.05）。热带念珠菌的PL和SAP酶活性均显著高于光滑念珠菌（P < 0.001）。
热带念珠菌分离株产生的这两种酶的量均显著高于光滑念珠菌分离株。
