墨西哥家庭饮用水中潜在致病性非结核分枝杆菌的出现：一项试点研究。
Occurrence of potentially pathogenic nontuberculous mycobacteria in Mexican household potable water: a pilot study.
作者信息
Perez-Martinez Iza, Aguilar-Ayala Diana A, Fernandez-Rendon Elizabeth, Carrillo-Sanchez Alma K, Helguera-Repetto Addy C, Rivera-Gutierrez Sandra, Estrada-Garcia Teresa, Cerna-Cortes Jorge F, Gonzalez-Y-Merchand Jorge A
机构信息
Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas-Instituto Politecnico Nacional, Prolongacion Carpio y Plan de Ayala S/N, Col, Casco de Santo Tomas, Delegacion Miguel Hidalgo, Mexico, D,F, CP 11340, Mexico.
出版信息
BMC Res Notes. 2013 Dec 11;6:531. doi: 10.1186/1756-0500-6-531.
BACKGROUND
Nontuberculous mycobacteria (NTM) are environmental opportunistic pathogens found in natural and human-engineered waters, including drinking water distribution systems and household plumbing. This pilot study examined the frequency of occurrence of NTM in household potable water samples in Mexico City. Potable water samples were collected from the "main house faucet" and kitchen faucet. The presence of aerobic-mesophilic bacteria (AMB), total coliforms (TC), fecal coliforms (FC) and NTM species were determined. Mycobacteria species were identified by PCR restriction enzyme pattern analysis (PRA) of the 65-kDa heat shock protein gene (hsp65) and sequencing of the hypervariable region 2 (V2) of the 16S rRNA gene and of the rpoB gene.
RESULTS
AMB (<100 CFU/ml) were present in 118 out of 120 samples; only two samples were outside guidelines ranges (>100 CFU/ml). TC and FC were detected in four and one samples, respectively. NTM species were recovered from 16% samples (19/120) and included M. mucogenicum (nine), M. porcinum (three), M. avium (three), M. gordonae (one), M. cosmeticum (one), M. fortuitum (one), and Mycobacterium sp (one). All household water samples that contained NTM complied with the standards required to grade the water as "good quality" potable water.
CONCLUSION
Household potable water may be a potential source of NTM infection in Mexico City.
背景
非结核分枝杆菌（NTM）是在天然水和人工水域中发现的环境机会致病菌，包括饮用水分配系统和家庭管道。这项初步研究调查了墨西哥城家庭饮用水样本中NTM的出现频率。从“主屋水龙头”和厨房水龙头采集饮用水样本。测定了需氧嗜温菌（AMB）、总大肠菌群（TC）、粪大肠菌群（FC）和NTM种类。通过对65 kDa热休克蛋白基因（hsp65）进行PCR限制性酶切图谱分析（PRA）以及对16S rRNA基因的高变区2（V2）和rpoB基因进行测序来鉴定分枝杆菌种类。
结果
120个样本中有118个存在AMB（<100 CFU/ml）；只有两个样本超出指导范围（>100 CFU/ml）。分别在4个和1个样本中检测到TC和FC。从16%的样本（19/120）中分离出NTM种类，包括产黏液分枝杆菌（9株）、猪分枝杆菌（3株）、鸟分枝杆菌（3株）、戈登分枝杆菌（1株）、美容分枝杆菌（1株）、偶然分枝杆菌（1株）和分枝杆菌属（1株）。所有含有NTM的家庭水样均符合将水评为“优质”饮用水所需的标准。
结论
在墨西哥城，家庭饮用水可能是NTM感染的潜在来源。
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