Department of Gastroenterology, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
Oncol Res. 2013;21(1):33-41. doi: 10.3727/096504013X13786659070316.
Oncogene Bmi-1 (B-cell-specific Moloney murine leukemia virus integration site 1) has attracted much attention for its involvement in the initiation of a variety of tumors. Our previous study showed that Bmi-1 was highly expressed in gastric cancer and correlated with patient prognosis. However, whether aberrant Bmi-1 expression was critical for the transformation of gastric epithelial cells remains unknown. In this study, we stably expressed Bmi-1 in a human gastric epithelial immortalized cell line, GES-1. The overexpression of Bmi-1 promoted cell growth and proliferation, inhibited apoptosis, enhanced clone formation capability, possessed the characteristics of anchorage-independent growth, and increased migration and invasion abilities. Therefore, our findings demonstrated that ectopic expression of Bmi-1 played an important role in the malignant transformation of gastric epithelial cells.
癌基因 Bmi-1（B 细胞特异性 Moloney 鼠白血病病毒整合位点 1）因其参与多种肿瘤的发生而备受关注。我们之前的研究表明，Bmi-1 在胃癌中高表达，并与患者预后相关。然而，Bmi-1 的异常表达是否对胃上皮细胞的转化至关重要尚不清楚。在本研究中，我们在人胃上皮永生化细胞系 GES-1 中稳定表达 Bmi-1。Bmi-1 的过表达促进细胞生长和增殖，抑制细胞凋亡，增强克隆形成能力，具有锚定非依赖性生长的特性，并增加迁移和侵袭能力。因此，我们的研究结果表明，Bmi-1 的异位表达在胃上皮细胞的恶性转化中发挥了重要作用。
