Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Rd., Hefei, 230022, Anhui, People's Republic of China.
Pathol Oncol Res. 2020 Jan;26(1):475-482. doi: 10.1007/s12253-019-00703-3. Epub 2019 Oct 22.
Long noncoding RNAs (lncRNAs) play important roles in regulating the apoptosis of gastric cancer (GC) cells. This study aims to investigate the underlying mechanism of lncRNA CASC9 in regulating the apoptosis of GC cells. The expressions of lncRNA and protein in GC tissues and cell lines were detected by qRT-PCR and western blot. GC cell apoptosis was detected by flow cytometry analysis. RNA pull-down and RNA immunoprecipitation (RIP) assays were conducted to verify the interaction between CASC9 and BMI1. LncRNA CASC9 was upregulated in GC tissue and GC cells, and high CASC9 expression was positively correlated with TNM stage and lymph node metastasis. Silencing CASC9 promoted the apoptosis of GC cells. LncRNA CASC9 could interact with BMI1 and positively regulate BMI1 expression. Silencing CASC9 promoted the ubiquitination of BMI1. In addition, lncRNA CASC9 regulated the apoptosis of GC cells through BMI1. Furthermore, interfering CASC9 inhibited the tumor growth of GC. LncRNA CASC9 could interact with BMI1 to regulate the degradation of BMI1, thus to affect the apoptosis of GC cells and suppressed tumor growth.
长链非编码 RNA（lncRNA）在调控胃癌（GC）细胞凋亡中发挥重要作用。本研究旨在探讨 lncRNA CASC9 在调控 GC 细胞凋亡中的作用机制。通过 qRT-PCR 和 Western blot 检测 GC 组织和细胞系中 lncRNA 和蛋白的表达。通过流式细胞术分析检测 GC 细胞凋亡。通过 RNA 下拉和 RNA 免疫沉淀（RIP）实验验证 CASC9 与 BMI1 之间的相互作用。lncRNA CASC9 在 GC 组织和 GC 细胞中上调，高表达 CASC9 与 TNM 分期和淋巴结转移呈正相关。沉默 CASC9 促进 GC 细胞凋亡。lncRNA CASC9 可与 BMI1 相互作用并正向调节 BMI1 表达。沉默 CASC9 促进 BMI1 的泛素化。此外，lncRNA CASC9 通过 BMI1 调节 GC 细胞的凋亡。此外，干扰 CASC9 抑制 GC 的肿瘤生长。lncRNA CASC9 可以与 BMI1 相互作用，调节 BMI1 的降解，从而影响 GC 细胞的凋亡并抑制肿瘤生长。
