Norman R I, Burgess A J, Allen E, Harrison T M
FEBS Lett. 1987 Feb 9;212(1):127-32. doi: 10.1016/0014-5793(87)81570-3.
Four monoclonal antibodies have been raised against voltage-sensitive Ca2+ channel dihydropyridine receptors from rabbit skeletal muscle. When tested by immunoblot assay of denatured transverse tubule membranes in reducing polyacrylamide gels, each recognised a single polypeptide of Mr approximately 140,000 that co-migrated with the large glycoprotein subunit of the purified receptor. In blots of nonreducing gels, a larger protein of Mr approximately 170,000 was seen and three of the antibodies recognised additional components at Mr approximately 310,000 and approximately 330,000. Crossreactive material of similar molecular mass was also seen in rabbit heart and brain, and in the skeletal muscle of other species.
已制备出四种针对兔骨骼肌电压敏感性Ca2+通道二氢吡啶受体的单克隆抗体。在还原聚丙烯酰胺凝胶中对变性横管膜进行免疫印迹分析时，每种抗体都识别出一条分子量约为140,000的单一多肽，该多肽与纯化受体的大糖蛋白亚基共同迁移。在非还原凝胶的印迹中，可见一条分子量约为170,000的更大蛋白质，其中三种抗体还识别出分子量约为310,000和330,000的其他成分。在兔心脏、脑以及其他物种的骨骼肌中也可见到类似分子量的交叉反应物质。
