Hussain I, Leibowitz M J
Gene. 1986;46(1):13-23. doi: 10.1016/0378-1119(86)90162-9.
A stable mRNA-dependent cell-free translation system from Saccharomyces cerevisiae, prepared by a modification of the method of Hofbauer et al. [Eur. J. Biochem. 122 (1982) 199-203] was active in translation of exogenous homologous and heterologous mRNAs. Optimal translational activity required the addition of polyamines and yeast tRNA. The m transcript of the M segment of double-stranded RNA, synthesized in vitro using the killer virus-associated RNA polymerase, directed the synthesis of preprotoxin polypeptide (M-p32), which was immunologically identified using antitoxin antibody. Sindbis virus capsid protein and rabbit globin were also translated from their mRNAs. Translation was inhibited by puromycin, sparsomycin and anisomycin. Analogues of the 5'-terminal caps present on most eukaryotic mRNA molecules inhibited translation of added mRNAs, including capped mRNAs and the uncapped killer virus mRNA.
通过对霍夫鲍尔等人[《欧洲生物化学杂志》122 (1982) 199 - 203]方法进行改进制备的来自酿酒酵母的稳定的依赖mRNA的无细胞翻译系统，对外源同源和异源mRNA的翻译具有活性。最佳翻译活性需要添加多胺和酵母tRNA。使用与杀伤病毒相关的RNA聚合酶体外合成的双链RNA M片段的m转录本，指导了前原毒素多肽（M - p32）的合成，该多肽使用抗毒素抗体进行了免疫鉴定。辛德毕斯病毒衣壳蛋白和兔珠蛋白也从它们的mRNA进行了翻译。翻译受到嘌呤霉素、稀疏霉素和茴香霉素的抑制。大多数真核mRNA分子上存在的5'-末端帽类似物抑制添加的mRNA的翻译，包括加帽的mRNA和未加帽的杀伤病毒mRNA。
