University of California, Santa Cruz Extension, 2505 Augustine Drive, Santa Clara, CA 95054, United States.
J Theor Biol. 2014 Apr 21;347:1-6. doi: 10.1016/j.jtbi.2013.12.001. Epub 2013 Dec 11.
The study of aging in fission bacteria has been especially difficult because of the challenge in identifying older sibling cells due to their morphological similarity to younger sibling cells. This study develops a generic method solely based on the analysis of cell growth rate (rate of cell volume increase). The proposed method does not require any special assumptions or measurements regarding the physical features of older siblings, such as older cell poles or slightly greater cell sizes. Therefore, the proposed method is applicable to the study of far more types of bacteria than those of existing methods. Bacteria that can be examined using this method include, but are not limited to, the following categories: (1) cocci bacteria in which tracking a larger number of old pole cells through successive generations is formidable, (2) possible bacteria where cell poles are either weakly correlated or uncorrelated to aging, and (3) bacteria that reproduce by multiple-fission. This new method provides a useful tool to study the relationship between cell poles and aging in bacteria.
由于难以识别由于形态相似而导致的年老细胞和年幼细胞，因此，研究裂变细菌的衰老特别困难。本研究开发了一种仅基于细胞生长速率（细胞体积增加率）分析的通用方法。该方法不需要关于年老细胞的物理特征（如老细胞极或稍大的细胞尺寸）的任何特殊假设或测量。因此，与现有方法相比，该方法适用于研究更多种类的细菌。可以使用该方法检查的细菌包括但不限于以下几类：（1）追踪多个连续世代的老极细胞非常困难的球菌细菌，（2）细胞极与衰老无关或弱相关的可能细菌，以及（3）通过多次裂变繁殖的细菌。这种新方法为研究细菌中细胞极与衰老之间的关系提供了有用的工具。
