Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech Republic.
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic.
FEBS Lett. 2014 Jan 31;588(3):443-9. doi: 10.1016/j.febslet.2013.11.038. Epub 2013 Dec 12.
We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids.
我们使用反式-Parinaric 酸（t-PnA）和 1,6-二苯基-1,3,5-己三烯（DPH）报告了活酿酒酵母质膜中与鞘脂有关的凝胶样微区的重组。与对照相比，鞘脂缺乏的 lcb1-100 突变体的膜中凝胶样域明显减少。鞘脂消耗也导致了同样的减少，这是由霉菌醇引起的。当通过外源性二氢神经酰胺绕过霉菌醇诱导的鞘脂生物合成阻断时，表型可以逆转。正如 DPH 荧光各向异性所记录的那样，随着鞘脂的消耗，较少有序的膜区域的脂质有序性也降低了。这些数据表明，侧向微区的组织是这些结构脂质的一个重要生理作用。
