Key Laboratory of Tuberculosis Prevention and Treatment of PLA, Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
Key Laboratory of Tuberculosis Prevention and Treatment of PLA, Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
Immunol Lett. 2014 Mar-Apr;158(1-2):66-72. doi: 10.1016/j.imlet.2013.12.003. Epub 2013 Dec 12.
Development of active TB is accompanied by immune suppression and the underlining mechanisms have been explored extensively in recent years. MDSCs are a heterogeneous group of immature and progenitor myeloid cells with strong immunosuppressive ability for both natural and adaptive immunity. In our analysis of CD244 (2B4)-expressing cells in PBMCs from patients with active TB, a CD3(-)CD244(high) subpopulation was identified. A match of cell population in flow cytometry showed that nearly all CD3(-)CD244(high) cells were CD3(-)HLA-DR(-)CD11b(int)CD33(+) cells. The CD3(-)CD244(high) cell population has phenotypes of CD3(-)CD19(-)CD56(-)CD15(-)CD66b(-)CD33(+)CD11b(+)CD14(-)HLA-DR(neg/low), which was consistent with MDSCs in humans as previously reported. Patients with active TB had higher frequencies of CD3(-)CD244(high) cells as compared with healthy controls. The CD3(-)CD244(high) cell population had high levels of NOS2 expression and was negatively correlated with activation and effective molecule production of CD4(+) and CD8(+) T cells. In conclusion, CD3(-)CD244(high) cells had phenotypes of MDSCs and CD244 might be used as a marker for human CD3(-)HLA-DR(-)CD11b(int)CD33(+) MDSCs.
活动性结核病的发展伴随着免疫抑制，近年来，其潜在机制已被广泛研究。髓系来源抑制细胞（MDSCs）是一群异质性的未成熟和祖细胞髓系细胞，对天然和适应性免疫具有强大的免疫抑制能力。在对活动性结核病患者 PBMC 中表达 CD244（2B4）的细胞进行分析时，我们鉴定出一个 CD3(-)CD244(high)亚群。流式细胞术匹配细胞群体显示，几乎所有的 CD3(-)CD244(high)细胞都是 CD3(-)HLA-DR(-)CD11b(int)CD33(+)细胞。CD3(-)CD244(high)细胞群体的表型为 CD3(-)CD19(-)CD56(-)CD15(-)CD66b(-)CD33(+)CD11b(+)CD14(-)HLA-DR(neg/low)，与之前报道的人类 MDSCs 一致。与健康对照组相比，活动性结核病患者的 CD3(-)CD244(high)细胞频率更高。CD3(-)CD244(high)细胞群体中 NOS2 的表达水平较高，与 CD4(+)和 CD8(+)T 细胞的活化和有效分子产生呈负相关。总之，CD3(-)CD244(high)细胞具有 MDSCs 的表型，CD244 可能被用作人类 CD3(-)HLA-DR(-)CD11b(int)CD33(+)MDSCs 的标志物。
