Khan Haroon, Khan Murad Ali, Hussain Sajid, Gaffar Rukhsana, Ashraf Nadeem
Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan
Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan.
Toxicol Ind Health. 2016 May;32(5):860-5. doi: 10.1177/0748233713513489. Epub 2013 Dec 12.
Natural healing agents are primarily focused to overcome unwanted side effects with synthetic drugs worldwide. In the proposed study, crude extracts and subsequent solvent fractions of Heliotropium strigosum were evaluated for antinociceptive and anticonvulsant activity in animal paradigms. In post acetic acid-induced writhing test, crude extract and fractions (hexane, ethyl acetate, and aqueous) demonstrated marked attenuation of nociception at test doses (50, 100, and 200 mg/kg i.p.). When challenged against thermally induced pain model, pretreatment of extracts exhibited prominent amelioration at test dose (50, 100, and 200 mg/kg i.p.). In both tests, inhibition of noxious stimulation was in a dose-dependent manner, and ethyl acetate fraction was most dominant. However, extracts did not antagonize the seizures and mortality induced by pentylenetetrazole. In conclusion, the extracts of H. strigosum illustrated significant antinociceptive effect in both centrally and peripherally acting pain models.
在全球范围内，天然治疗剂主要致力于克服合成药物产生的不良副作用。在本研究中，对硬毛天芥菜的粗提物及其后续溶剂萃取物进行了动物模型的抗伤害感受和抗惊厥活性评估。在乙酸诱导的扭体试验中，粗提物及其萃取物（己烷、乙酸乙酯和水相）在试验剂量（腹腔注射50、100和200mg/kg）下均表现出明显的伤害感受减弱。当针对热诱导疼痛模型进行测试时，提取物预处理在试验剂量（腹腔注射50、100和200mg/kg）下表现出显著的改善作用。在这两种试验中，对有害刺激的抑制均呈剂量依赖性，且乙酸乙酯萃取物的作用最为显著。然而，提取物并未拮抗戊四氮诱导的癫痫发作和死亡。总之，硬毛天芥菜提取物在中枢和外周作用的疼痛模型中均表现出显著的抗伤害感受作用。
