Rauf Abdur, Khan Rehan, Khan Haroon, Ullah Barkat, Pervez Samreen
Department of Pharmacy, Abdul Wali Khan University, Mardan 23200, Pakistan.
BMC Complement Altern Med. 2014 Nov 18;14:448. doi: 10.1186/1472-6882-14-448.
Fever and pain management is a very challenging job for the clinician as the available synthetic agents are causing serious side effects. The present research article deals with the antipyretic and antinociceptive activity of extract/fractions of Potentilla evestita and acacetin isolated from the chloroform fraction of the plant.
Various chromatographic and spectroscopic techniques were used for the isolation and characterizion of compound. In-vivo yeast induced fibrile mice were used for antipyretic activity while acetic acid induced writhing and formalin tests were used for antinociceptive.
The extract/fractions of P. evestita caused marked antipyretic effect during various assessment times in which chloroform was the most prominent followed by ethyl acetate. When acacetin was injected, it produced marked effect with maximum activity of 33.28% and 55.01% at 5 and 10 mg/kg i.p respectively. When studied in acetic acid induced writhing test, the extract/fractions evoked significant antinociceptive effect in which chloroform was the most effective fraction followed by ethyl acetate. Acacetin showed significant antinociceptive effect with 44.77% and 67.03% reduction in abdominal constriction at 5 and 10 mg/kg i.p., respectively. Similarly, it evoked significant dose dependent reduction in noxious stimulation with 42.07% and 64.57% pain attenuation at 5 and 10 mg/kg i.p., respectively in initial phase. In the late phase, it illustrated more dominant effect with 46.32% and 67.29% reduction of painful sensation.
In conclusion, the extract/fractions of P. evestita as well as the isolated compound, acacetin showed strong antipyretic and antinociceptive activity in various animal models possibly mediated through both peripheral and central mechanism.
发热和疼痛管理对临床医生来说是一项极具挑战性的工作，因为现有的合成药物会产生严重的副作用。本研究文章探讨了委陵菜提取物/馏分以及从该植物氯仿馏分中分离出的刺槐素的解热和抗伤害感受活性。
采用各种色谱和光谱技术对化合物进行分离和表征。用体内酵母诱导的发热小鼠进行解热活性实验，用醋酸诱导扭体和福尔马林试验进行抗伤害感受实验。
委陵菜提取物/馏分在不同评估时间均产生显著的解热作用，其中氯仿馏分最为显著，其次是乙酸乙酯馏分。注射刺槐素时，它产生了显著效果，腹腔注射5和10mg/kg时最大活性分别为33.28%和55.01%。在醋酸诱导扭体试验中研究时，提取物/馏分产生了显著的抗伤害感受作用，其中氯仿馏分是最有效的馏分，其次是乙酸乙酯馏分。刺槐素显示出显著的抗伤害感受作用，腹腔注射5和10mg/kg时腹部收缩分别减少44.77%和67.03%。同样，在初始阶段，它在腹腔注射5和10mg/kg时分别引起有害刺激的显著剂量依赖性降低，疼痛减轻42.07%和64.57%。在后期阶段，它表现出更显著的效果，疼痛感觉减少46.32%和67.29%。
总之，委陵菜提取物/馏分以及分离出的化合物刺槐素在各种动物模型中均显示出强大的解热和抗伤害感受活性，可能通过外周和中枢机制介导。
