Regional Laboratory of Public Health, Medical School, Heraklion, Crete, Greece.
Laboratory of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, Voutes - Staurakia, Medical School, University of Crete, 71110, Heraklion, Crete, Greece E-mail:
J Water Health. 2013 Dec;11(4):762-71. doi: 10.2166/wh.2013.015.
In Greece, standard tests are performed in watering and cooling systems of hotels. A total of 1,494 water samples were collected during 2004-2011 from 124 hotels from four regions in Crete (Greece). Samples were tested for the presence of Legionella spp.; 103 isolates were identified and typed by polymerase chain reaction (PCR)-sequencing and sequence-based typing (SBT) (in case of L. pneumophila sg 1). Of those, 48 belonged to various serogroups of L. pneumophila (sg 1, 2, 3, 5, 6, 8, 12, 13, and 15), 32 were characterized as L. anisa, 17 as L. taurinensis and there was a single occurrence of L. quinlivanii, L. maceachernii, and L. oakridgensis. In the case of L. pneumophila SG1, one prevalent sequence type was revealed (ST37). The variability of Legionella spp. observed questions the existence of a single ST of the L. pneumophila sg1 species and leads towards the need for a genetic level investigation of all Legionnaires' disease cases.
在希腊，对酒店的供水和冷却系统进行标准测试。2004 年至 2011 年期间，从克里特岛（希腊）四个地区的 124 家酒店共采集了 1494 个水样。对水样进行军团菌属检测；通过聚合酶链反应（PCR）测序和基于序列的分型（SBT）（针对 L. pneumophila sg 1）对 103 个分离株进行鉴定和分型。其中，48 株属于各种血清型的 L. pneumophila（sg 1、2、3、5、6、8、12、13 和 15），32 株为 L. anisa，17 株为 L. taurinensis，仅有一株为 L. quinlivanii、L. maceachernii 和 L. oakridgensis。对于 L. pneumophila SG1，揭示了一个普遍的序列型（ST37）。观察到的军团菌属的变异性质疑了 L. pneumophila sg1 物种的单一 ST 的存在，并促使对所有军团病病例进行遗传水平的调查。
