Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185, Rome, Italy,
Anal Bioanal Chem. 2014 Feb;406(5):1423-35. doi: 10.1007/s00216-013-7549-y. Epub 2013 Dec 15.
Salinity is one of the major abiotic stress conditions limiting crop growth and productivity. Duilio is a wheat genotype that shows tolerant behavior in both salt-stress and drought-stress conditions. Toward better understanding of the biochemical response to salinity in this genotype of durum wheat, a comparative label-free shotgun proteomic analysis based on normalized spectral abundance factors was conducted on wheat leaf samples subjected to increasing salt-stress levels (100 and 200 mmol L(-1) NaCl) with respect to untreated samples. We found significant changes in 71 proteins for the first stress level, in 83 proteins at the higher salinity level, and in 88 proteins when comparing salt-stress levels with each other. The major changes concerned the proteins involved in primary metabolism and production of energy, followed by those involved in protein metabolism and cellular defense mechanisms. Some indications of different specific physiological and defense mechanisms implicated in increasing tolerance were obtained. The enhanced salinity tolerance in Duilio appeared to be governed by a higher capacity for osmotic homeostasis, a more efficient defense, and an improvement of protection from mechanical stress by increased cell wall lignifications, allowing a better potential for growth recovery.
盐度是限制作物生长和生产力的主要非生物胁迫条件之一。Duilio 是一种小麦基因型，在盐胁迫和干旱胁迫条件下都表现出耐受行为。为了更好地理解该硬质小麦基因型对盐度的生化反应，我们对小麦叶片样本进行了基于归一化谱丰度因子的无标记 shotgun 蛋白质组学分析，这些样本分别在受到不同盐度胁迫水平（100 和 200mmol/L NaCl）和未处理样本的情况下进行了比较。我们发现，在第一个胁迫水平下，有 71 种蛋白质发生了显著变化，在较高盐度水平下有 83 种蛋白质发生了变化，在比较盐度水平时，有 88 种蛋白质发生了变化。主要变化涉及到参与初级代谢和能量产生的蛋白质，其次是参与蛋白质代谢和细胞防御机制的蛋白质。获得了一些与增加耐受性相关的不同特定生理和防御机制的迹象。Duilio 增强的耐盐性似乎是由更高的渗透平衡能力、更有效的防御以及通过增加细胞壁木质化来改善对机械胁迫的保护所控制的，这使得其具有更好的生长恢复潜力。
