阿魏提取物及其成分伞形花内酯对豚鼠气管平滑肌的舒张作用。

Relaxant effects of asafoetida extract and its constituent umbelliprenin on guinea-pig tracheal smooth muscle.

作者信息

Bayrami Goltaj, Boskabady Mohammad Hossein, Iranshahi Mehrdad, Gholamnezhad Zahra

机构信息

Department of Physiology and Pharmaceutical Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, 9177948564, Iran.

出版信息

Chin J Integr Med. 2013 Dec 12. doi: 10.1007/s11655-013-1550-3.

DOI:10.1007/s11655-013-1550-3

PMID:24338082

Abstract

OBJECTIVE

To investigate the relaxant effects of the asafoetida (Ferula Asafoetida Oleo-Gum-Resin) and its coumarin constituent umbelliprenin on tracheal chains of guinea pigs.

METHODS

The relaxant effects of three cumulative concentrations of the aqueous extract (2, 5 and 10 mg/mL), umbelliprenin (0.04, 0.2 and 0.4 mg/mL), theophylline (0.05, 0.1 and 0.15 mg/mL) and saline were examined by their relaxant effects on precontracted tracheal chains of guinea pig by 60 mmol/L KCl (group 1), and 10 μ mol/L methacholine (group 2).

RESULTS

In group 1 all concentrations of theophylline and the highest concentration of the extract showed signififi cant relaxant effects compared with that of saline (P<0.01 for theophylline and P<0.05 for extract). In group 2, relaxant effects of all concentrations of theophylline, extract and two higher concentrations of umbelliprenin differed significantly compared with saline [P<0.01 for all cases except low concentration of umbelliprenin (0.04 mg/mL)]. There were no signififi cant differences between relaxant effects of the extract and theophylline in group 2. The relaxant effect of the extract in group 2 was signififi cantly greater than that of group 1 (P<0.01). The relaxant effect of the extract was significantly more potent than umbelliprenin in all groups (P<0.05 or P<0.01).

CONCLUSION

These results showed a potent relaxant effect for the asafoetida extract on tracheal smooth muscle which is, at least, in part, due to its constituent umbelliprenin. A muscarinic receptor blockade was also suggested for the extract.

摘要

目的

研究阿魏（阿魏油胶树脂）及其香豆素成分伞形前胡素对豚鼠气管链的舒张作用。

方法

通过观察三种累积浓度的水提取物（2、5和10mg/mL）、伞形前胡素（0.04、0.2和0.4mg/mL）、茶碱（0.05、0.1和0.15mg/mL）和生理盐水对60mmol/L氯化钾预收缩的豚鼠气管链（第1组）和10μmol/L乙酰甲胆碱预收缩的豚鼠气管链（第2组）的舒张作用，来检测它们的舒张效果。

结果

在第1组中，与生理盐水相比，所有浓度的茶碱和最高浓度的提取物均显示出显著的舒张作用（茶碱P<0.01，提取物P<0.05）。在第2组中，与生理盐水相比，所有浓度的茶碱、提取物和两种较高浓度的伞形前胡素的舒张作用均有显著差异[除低浓度伞形前胡素（0.04mg/mL）外，所有情况P<0.01]。第2组中提取物和茶碱的舒张作用之间无显著差异。第2组中提取物的舒张作用显著大于第1组（P<0.01）。在所有组中，提取物的舒张作用均显著强于伞形前胡素（P<0.05或P<0.01）。