Boskabady M H, Aslani M R, Kiani S
Department of Physiology, Ghaem Medical Centre, Mashhad University of Medical Sciences, Mashhad, Iran.
Phytother Res. 2006 Jan;20(1):28-33. doi: 10.1002/ptr.1796.
Thymus vulgaris for the treatment of respiratory diseases is indicated widely, and relaxant effects on smooth muscle have been shown previously. In the present study, the relaxant effects of macerated and aqueous extracts of Thymus vulgaris on tracheal chains of guinea-pigs were examined using cumulative concentrations of macerated and aqueous extracts in comparison with saline (as the negative control) and theophylline (as the positive control). The relaxant effects of four cumulative concentrations of macerated and aqueous extracts (0.25, 0.5, 0.75 and 1.0 g %) in comparison with saline (as the negative control) and four cumulative concentrations of theophylline (0.25, 0.5, 0.75 and 1.0 mm; as the positive control) were examined for their relaxant effects on precontracted tracheal chains of guinea-pig by 60 mm KCl and 10 microm methacholine in two different conditions: non-incubated tissues and incubated tissues with 1 microm propranolol and 1 microm chlorphenamine. There were significant correlations between the relaxant effects and the concentrations for both extracts and theophylline in all experimental groups (p < 0.01 to p < 0.001). These results demonstrated a potent relaxant effect of Thymus vulgaris on guinea-pig tracheal chains that was comparable to theophylline at the concentrations used.
百里香广泛用于治疗呼吸系统疾病，此前已显示出对平滑肌的松弛作用。在本研究中，使用浸软提取物和水提取物的累积浓度，与生理盐水（作为阴性对照）和茶碱（作为阳性对照）相比，检测了百里香浸软提取物和水提取物对豚鼠气管链的松弛作用。在两种不同条件下，检测了浸软提取物和水提取物的四个累积浓度（0.25、0.5、0.75和1.0 g%）与生理盐水（作为阴性对照）以及茶碱的四个累积浓度（0.25、0.5、0.75和1.0 mM；作为阳性对照）对60 mM氯化钾和10 μM乙酰甲胆碱预收缩的豚鼠气管链的松弛作用：未孵育组织以及用1 μM普萘洛尔和1 μM氯苯那敏孵育的组织。在所有实验组中，提取物和茶碱的松弛作用与浓度之间均存在显著相关性（p < 0.01至p < 0.001）。这些结果表明，在所使用的浓度下，百里香对豚鼠气管链具有强大的松弛作用，与茶碱相当。
