Khaghanzadeh Narges, Samiei Afshin, Mojtahedi Zahra, Ramezani Mohammad, Hosseinzadeh Massood, Ghaderi Abbas
Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Department of Immunology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Iran J Basic Med Sci. 2017 Jul;20(7):829-834. doi: 10.22038/IJBMS.2017.9021.
Umbelliprenin is a prenyloxy-coumarin with pharmacologically polyvalent activity. Several studies have shown Several studies have been shown its anti-inflammatory, anti-tumor, antioxidant, and antigenotoxic activities. However, the exact mechanism of action of this compound on the immune response has not yet been shown. Here, we investigated umbelliprenin effects on the predominance of Th1 and Th2 responses in normal C57/BL6 mice.
Umbelliprenin (2.5 mg/200 µl IP) were administered to six C57/BL6 mice every other day for 8 days. Paraffin and PBS-injected mice were enrolled as solvent and control groups, respectively (n=6 mice/group). IL-10, IFN-γ, and IL-4 levels were determined in sera and also in splenocytes culture supernatants in the presence of Con A (3 µg/ml) after 72 hr. H&E staining of paraffin embedded blocks was performed for lung and liver tissues of mice.
Umbelliprenin could significantly increase the secretion of IFN-γ and IL-4 in sera and IL-10 in splenocytes cultures. Comparison of IFN-γ/IL-4 in the sera and splenocytes culture supernatants showed lower ratios in umbelliprenin treated mice than in solvent and untreated groups.
The study showed that umbelliprenin could induce anti-inflammatory responses via the predominance of Th2 cells and some regulatory responses in C57/BL6 mice.
伞形前胡素是一种具有多种药理活性的异戊烯氧基香豆素。多项研究已表明其具有抗炎、抗肿瘤、抗氧化和抗基因毒性活性。然而，该化合物对免疫反应的确切作用机制尚未明确。在此，我们研究了伞形前胡素对正常C57/BL6小鼠Th1和Th2反应优势的影响。
每隔一天给6只C57/BL6小鼠腹腔注射伞形前胡素（2.5mg/200μl），共注射8天。分别将注射石蜡和PBS的小鼠作为溶剂对照组和空白对照组（每组n = 6只小鼠）。72小时后，在有刀豆蛋白A（3μg/ml）存在的情况下，测定血清以及脾细胞培养上清液中的IL-10、IFN-γ和IL-4水平。对小鼠的肺和肝组织进行石蜡包埋块的苏木精-伊红染色。
伞形前胡素可显著增加血清中IFN-γ和IL-4的分泌以及脾细胞培养物中IL-10的分泌。血清和脾细胞培养上清液中IFN-γ/IL-4的比较显示，伞形前胡素处理组小鼠的该比值低于溶剂对照组和未处理组。
该研究表明，伞形前胡素可通过Th2细胞优势及一些调节反应在C57/BL6小鼠中诱导抗炎反应。
