Fakhar-e-Alam Muhammad, Kishwar S, Willander M
Department of Physics, GC University, Faisalabad, Pakistan,
Lasers Med Sci. 2014 May;29(3):1189-94. doi: 10.1007/s10103-013-1501-4. Epub 2013 Dec 11.
Cytotoxic effects of zinc oxide (ZnO) nanomaterials, individual and conjugated with a photosensitizer (protoporphyrin IX), were studied in the presence and absence of ultraviolet light exposure (240 nm of light wavelength for a very short time exposure) in cell cultures of human normal and cancerous skin models. Zinc Oxide nanowires (ZnO NWs) were grown on the capillary tip and conjugated with protoporphyrin IX (PpIX). This coated tip was used as tool/pointer for intracellular drug delivery protocol in suggested normal as well as carcinogenic cellular models. After true delivery of optimal drug, the labelled biological model was irradiated with UV-A, which led to a loss of mitochondrial membrane potential, as tested by neutral red assay (NRA).
在人类正常和癌性皮肤模型的细胞培养中，研究了氧化锌（ZnO）纳米材料单独以及与光敏剂（原卟啉IX）结合时，在有和没有紫外线照射（240nm波长的光，极短时间照射）情况下的细胞毒性作用。氧化锌纳米线（ZnO NWs）生长在毛细管尖端，并与原卟啉IX（PpIX）结合。这种包被的尖端被用作在建议的正常以及致癌细胞模型中进行细胞内药物递送方案的工具/指针。在真正递送最佳药物后，用UV-A照射标记的生物学模型，通过中性红试验（NRA）测试，这导致线粒体膜电位丧失。
